Evidence map›Paper›PMID 42445515›Full record

ArticleChemical science2026

The development of activity-based mannanase probes.

Massimo Tedeschi, Vincent A J Lit, Nicholas G S McGregor, Tessa Gote, Prajeesh Kooloth Valappil, Mark Arentshorst, Bogdan I Florea, Berend Gagestein, Zachary Armstrong, Jeroen D C Codée and 5 more

Abstract read
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In one paragraph

Article in Chemical science, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors.

Massimo TedeschiLeiden Institute of Chemistry, Leiden University Einsteinweg 55 2300 RA Leiden The Netherlands h.s.overkleeft@lic.leidenuniv.nl.ORCID https://orcid.org/0000-0003-0229-7103
Vincent A J LitLeiden Institute of Chemistry, Leiden University Einsteinweg 55 2300 RA Leiden The Netherlands h.s.overkleeft@lic.leidenuniv.nl.ORCID https://orcid.org/0000-0001-9134-894X
Nicholas G S McGregorYork Structural Biology Laboratory, Department of Chemistry, The University of York Heslington York YO10 5DD UK.ORCID https://orcid.org/0000-0003-1075-7368
Tessa GoteLeiden Institute of Chemistry, Leiden University Einsteinweg 55 2300 RA Leiden The Netherlands h.s.overkleeft@lic.leidenuniv.nl.
Prajeesh Kooloth ValappilInstitute of Biology Leiden, Leiden University Sylviusweg 72 2333 BE Leiden The Netherlands.ORCID https://orcid.org/0000-0002-6402-7697
Mark ArentshorstInstitute of Biology Leiden, Leiden University Sylviusweg 72 2333 BE Leiden The Netherlands.
Bogdan I FloreaLeiden Institute of Chemistry, Leiden University Einsteinweg 55 2300 RA Leiden The Netherlands h.s.overkleeft@lic.leidenuniv.nl.ORCID https://orcid.org/0000-0001-7114-2266
Berend GagesteinLeiden Institute of Chemistry, Leiden University Einsteinweg 55 2300 RA Leiden The Netherlands h.s.overkleeft@lic.leidenuniv.nl.ORCID https://orcid.org/0000-0002-0993-6812
Zachary ArmstrongLeiden Institute of Chemistry, Leiden University Einsteinweg 55 2300 RA Leiden The Netherlands h.s.overkleeft@lic.leidenuniv.nl.ORCID https://orcid.org/0000-0002-4086-2946
Jeroen D C CodéeLeiden Institute of Chemistry, Leiden University Einsteinweg 55 2300 RA Leiden The Netherlands h.s.overkleeft@lic.leidenuniv.nl.ORCID https://orcid.org/0000-0003-3531-2138
Alba Nin-HillDepartment de Química Inorgànica i Orgànica & IQTCUB, Universitat de Barcelona Barcelona 08028 Spain.ORCID https://orcid.org/0000-0002-8670-9031
Carme RoviraDepartment de Química Inorgànica i Orgànica & IQTCUB, Universitat de Barcelona Barcelona 08028 Spain.ORCID https://orcid.org/0000-0003-1477-5010
Arthur F J RamInstitute of Biology Leiden, Leiden University Sylviusweg 72 2333 BE Leiden The Netherlands.ORCID https://orcid.org/0000-0002-2487-8016
Gideon J DaviesYork Structural Biology Laboratory, Department of Chemistry, The University of York Heslington York YO10 5DD UK.ORCID https://orcid.org/0000-0002-7343-776X
Herman S OverkleeftLeiden Institute of Chemistry, Leiden University Einsteinweg 55 2300 RA Leiden The Netherlands h.s.overkleeft@lic.leidenuniv.nl.ORCID https://orcid.org/0000-0001-6976-7005

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

β-Mannanases are endo-acting glycoside hydrolases (GHs) that cleave β-1,4 glycosidic linkages in mannan-rich plant cell wall polysaccharides. They find application in the food and paper industries. Activity-based probes (ABPs) are powerful tools for GH profiling in complex biological samples, yet to date, bespoke ABPs reporting on mannanases have not been reported. Here, we describe the synthesis of cyclophellitol-inspired ABPs based on mannobiose, mannotriose, and glucomannose, and their use in reporting mannanase activities in secretomes from saprophytic bacteria and fungi grown on mannan-containing biomass polysaccharides. In addition to mannanases, our ABPs also labelled cellulases in secretomes from both

Identifiers

PMID42445515
PMCPMC13358891

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.