ArticleFrontiers in pharmacology2026
A curriculum-integrated learning experience linking experimental pharmacology, cell culture and Nrf2-related gene expression analysis in undergraduate biotechnology education.
Article in Frontiers in pharmacology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Background: Undergraduate biotechnology education requires integrated, experiential approaches that help students connect pharmacological modulation, cellular models, molecular analysis and data interpretation within coherent biological problems. This study describes and evaluates a curriculum-integrated learning experience linking experimental pharmacology, cell culture and Nrf2-related gene expression analysis in third-year Biotechnology students. Methods: A total of 32 students participated in the survey-based evaluation. The activity used A549 cells, sulforaphane as an Nrf2-activating compound and tert-butyl hydroperoxide as a pro-oxidant stimulus. Students followed an integrated workflow combining treatment design, cell culture handling, Nrf2-related gene selection, PCR-based gene expression analysis and scientific writing. Perceived learning was assessed using paired pre-post Likert-scale items and analyzed with the Wilcoxon matched-pairs signed-rank test. Post-intervention perceptions, satisfaction and open-ended feedback were analyzed descriptively. Results: Students showed significant improvements in all five pre-post items assessing perceived confidence or familiarity ( Conclusion: This curriculum-integrated learning experience was associated with perceived gains across pharmacological, cellular and molecular components of a biotechnology workflow. The intervention may provide a transferable model for integrating experiential, research-oriented and competency-based learning in undergraduate biotechnology education.
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