Evidence map›Paper›PMID 42445219›Full record

ArticleIJTLD open2026

Refining cytokine signatures: flow cytometry-based differentiation between TB infection and disease.

K Doležalová, J Goliáš, M Ibrahimová, L Bača, E Kopecká, I Hriciková, S Rajnochová-Bloudíčková, T Philipp, M Koziar Vašáková

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Article in IJTLD open, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

9 authors.

K DoležalováDepartment of Paediatrics of the First Faculty of Medicine, Thomayer University Hospital, Charles University, Prague, Czech Republic.
J GoliášLaboratory of Immunology, Thomayer University Hospital, Prague, Czech Republic.
M IbrahimováLaboratory of Immunology, Thomayer University Hospital, Prague, Czech Republic.
L BačaDepartment of Paediatrics of the First Faculty of Medicine, Thomayer University Hospital, Charles University, Prague, Czech Republic.
E KopeckáDepartment of Respiratory Medicine of the First Faculty of Medicine Charles University, Thomayer University Hospital, Prague, Czech Republic.
I HricikováDepartment of Respiratory Medicine of the First Faculty of Medicine Charles University, Thomayer University Hospital, Prague, Czech Republic.
S Rajnochová-BloudíčkováTransplantation Centre, Nephrology Department, Institute for Clinical and Experimental Medicine, Prague, Czech Republic.
T PhilippDepartment of Rheumatology and Physiotherapy, Thomayer University Hospital, Third Faculty of Medicine Charles University, Prague, Czech Republic.
M Koziar VašákováDepartment of Respiratory Medicine of the First Faculty of Medicine Charles University, Thomayer University Hospital, Prague, Czech Republic.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundDistinguishing TB infection (TBI) from TB disease remains difficult because routine tests, including tuberculin skin test and interferon-gamma release assay (IGRA), cannot separate these states. We assessed a flow cytometry-based assay that measures T-cell cytokine responses after stimulation with

methodsWe enrolled 118 IGRA-positive participants, including individuals with TB/TBI. Individuals were grouped by age and disease category into paediatric/adult TB, TBI, and immunocompromised TBI. Peripheral blood lymphocytes were stimulated with ESAT-6/CFP-10 antigens under CD4

resultsAmong children, most cytokine responses overlapped between TBI and TB, except TNF-α in CD8

conclusionFlow-cytometric cytokine profiling may help distinguish TBI from TB, with age-specific markers. TNF-α in CD8

Indexed as

flow cytometry–based assayIGRAimmunityinterferon-gamma release assaytuberculosis

Identifiers

PMID42445219
PMCPMC13362205

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.