Evidence map›Paper›PMID 42444595›Full record

ArticleJournal of mass spectrometry : JMS2026

IP-LC-MS/MS-Based Identification of Candidate TPL2 (MAP3K8)-Associated Proteins in Porcine PK-15 Cells.

Dajun Zhang, Dengshuai Zhao, Jiajun Lin, Shikai Cai, Yuanhang Zhang, Ping Li, Han Gao, Limei Qin, Keshan Zhang

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of mass spectrometry : JMS, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
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0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Dajun ZhangSchool of Animal Science and Technology, Foshan University, Foshan, China.ORCID https://orcid.org/0009-0003-5245-7168
Dengshuai ZhaoSchool of Animal Science and Technology, Foshan University, Foshan, China.ORCID https://orcid.org/0009-0008-2613-0598
Jiajun LinSchool of Animal Science and Technology, Foshan University, Foshan, China.
Shikai CaiSchool of Animal Science and Technology, Foshan University, Foshan, China.
Yuanhang ZhangSchool of Animal Science and Technology, Foshan University, Foshan, China.
Ping LiSchool of Animal Science and Technology, Foshan University, Foshan, China.ORCID https://orcid.org/0009-0007-9149-0939
Han GaoSchool of Animal Science and Technology, Foshan University, Foshan, China.
Limei QinSchool of Animal Science and Technology, Foshan University, Foshan, China.
Keshan ZhangSchool of Animal Science and Technology, Foshan University, Foshan, China.

Funding

Basic and Applied Basic Research Foundation of Guangdong Province 2024A1515140152Foshan University CGZ07001Guangdong Basic and Applied Basic Research FoundationGuangdong Provincial Department of Agriculture and Rural Affairs 2024CXTD15National Key Research and Development Program Projects
6 · The paper itself

Abstract

Tumor progression locus 2 (TPL2, also known as MAP-3K8/COT) is a kinase that regulates MAPK and NF-κB signaling and contributes to antiviral immunity. While mammalian TPL2 has been widely studied, the TPL2-associated protein profile in swine cells remains largely unexplored. In this study, we generated an IP-LC-MS/MS-based candidate dataset of TPL2-associated proteins in porcine kidney epithelial (PK-15) cells. FLAG-tagged TPL2 was overexpressed in PK-15 cells and enriched by immunoprecipitation, followed by liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis. After subtraction of proteins detected in the empty-vector negative IP control, a total of 666 candidate TPL2-associated proteins were identified. Bioinformatic analyses, including Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment, and protein-protein interaction (PPI) network analysis, indicated that these candidates were mainly associated with RNA binding, protein modification, ubiquitin-proteasome processes, spliceosome-related pathways, and immune-associated signaling. Collectively, our results provide an IP-LC-MS/MS-based candidate resource of TPL2-associated proteins in porcine PK-15 cells, offering a useful basis for future studies on TPL2-mediated host signaling and virus-host interactions.

Indexed as

MAP Kinase Kinase KinasesProto-Oncogene ProteinsAnimalsCell LineImmunoprecipitationKidneyLiquid Chromatography-Mass SpectrometryProtein Interaction MapsSwineTandem Mass SpectrometryMAP Kinase Kinase KinasesProto-Oncogene Proteinscandidate associated proteinsIP‐MS/MSPK‐15 cellsproteomeTPL2

Identifiers

PMID42444595
PMCPMC13366451

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.