ArticleScientific reports2026
Discovery and quantification of novel diazepam metabolites BP-246 and BP-271 in freshwater fish using HRMS and UPLC-MS/MS.
Article in Scientific reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors.
Funding
Abstract
Diazepam, commonly used in freshwater fish transport and aquaculture, poses potential food safety and public health risks due to its residues and metabolites. However, current monitoring efforts primarily focus on the parent drug and its major metabolites, leaving potential toxic transformation products uncharacterized. This study reports two novel metabolites (BP-246, 5-chloro-2-(methylamino)phenyl)(phenyl)methanone, and BP-271, 6-chloro-1-methyl-4-phenylquinazolin-2(1 H)-one) in freshwater fish muscle, a matrix in which these compounds have not been previously reported, using liquid chromatography-Orbitrap high-resolution mass spectrometry (LC-Orbitrap HRMS). Using ProTox3.0 and ADMETlab3.0 for toxicity prediction revealed that BP-246 and BP-271 exhibit high blood-brain barrier permeability, neurotoxicity and ecotoxicity. A sensitive and validated ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method was developed for the simultaneous quantification of diazepam, three established metabolites (nordiazepam, oxazepam, temazepam), and the two novel compounds in fish tissue. Sample preparation involved acetonitrile ultrasonication extraction followed by solid-phase extraction (SPE) purification. Matrix-matched calibration curves demonstrated excellent linearity ( R
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.