Evidence map›Paper›PMID 42437970›Full record

ArticleThe New phytologist2026

A hickory diacylglycerol acyltransferase gene coordinates fatty acid metabolism and aluminum tolerance in plants.

Shan Zheng, Wenchao Chen, Jiaqi Zhang, Fengmin Wang, Jiamin Zhao, Baoxin Zhang, Xinying Zhou, Qixiang Zhang, Jiasheng Wu, Heqiang Lou

Abstract read
In one paragraph

Article in The New phytologist, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Shan ZhengState Key Laboratory for Development and Utilization of Forest Food Resources, Zhejiang A&F University, Hangzhou, 311300, China.ORCID https://orcid.org/0009-0008-0483-3102
Wenchao ChenState Key Laboratory for Development and Utilization of Forest Food Resources, Zhejiang A&F University, Hangzhou, 311300, China.ORCID https://orcid.org/0000-0003-1695-2877
Jiaqi ZhangState Key Laboratory for Development and Utilization of Forest Food Resources, Zhejiang A&F University, Hangzhou, 311300, China.
Fengmin WangState Key Laboratory for Development and Utilization of Forest Food Resources, Zhejiang A&F University, Hangzhou, 311300, China.
Jiamin ZhaoState Key Laboratory for Development and Utilization of Forest Food Resources, Zhejiang A&F University, Hangzhou, 311300, China.
Baoxin ZhangState Key Laboratory for Development and Utilization of Forest Food Resources, Zhejiang A&F University, Hangzhou, 311300, China.
Xinying ZhouState Key Laboratory for Development and Utilization of Forest Food Resources, Zhejiang A&F University, Hangzhou, 311300, China.
Qixiang ZhangState Key Laboratory for Development and Utilization of Forest Food Resources, Zhejiang A&F University, Hangzhou, 311300, China.ORCID https://orcid.org/0000-0002-6657-5101
Jiasheng WuState Key Laboratory for Development and Utilization of Forest Food Resources, Zhejiang A&F University, Hangzhou, 311300, China.ORCID https://orcid.org/0000-0002-2515-4677
Heqiang LouState Key Laboratory for Development and Utilization of Forest Food Resources, Zhejiang A&F University, Hangzhou, 311300, China.ORCID https://orcid.org/0000-0002-2454-098X

Funding

National Natural Science Foundation of China 32422061; 32271923Scientific Research Startup Fund Project of Zhejiang A&F University 2025LFR033
6 · The paper itself

Abstract

Triacylglycerol (TAG) accumulation and abiotic stress tolerance are critical for woody oil crop productivity, yet their coordination remains poorly understood. Here, we characterized CcDGAT1, a diacylglycerol acyltransferase 1 gene from hickory (Carya cathayensis) that links oil accumulation with aluminum (Al) tolerance. CcDGAT1 was highly expressed in developing kernels and vegetative tissues and was strongly induced by Al stress. CcDGAT1 overexpression increased total oil content and unsaturated fatty acid levels and enhanced Al tolerance, as shown by improved membrane integrity, root growth, and biomass. Under Al stress, CcDGAT1 overexpression promoted the accumulation of 18:2/18:3-containing TAGs while reducing 18:2/18:3-enriched membrane lipid pools. Consistent with this protective role, transient silencing of CcDGAT1 in hickory roots aggravated Al-induced lipid peroxidation and electrolyte leakage. Dual-luciferase, yeast one-hybrid, and electrophoretic mobility shift assays demonstrated that CcATML1 and CcWRKY11a directly activate CcDGAT1 transcription. Overexpression of these transcription factors increased CcDGAT1 expression, fatty acid content, membrane integrity, and Al tolerance. Together, these findings establish CcDGAT1 as a functional oil-promoting DGAT1 in hickory and support a model in which CcDGAT1-associated lipid remodeling contributes to Al-stress protection. The CcATML1/CcWRKY11a-CcDGAT1 module further provides a transcriptional framework for linking lipid metabolism with stress resilience in woody oil crops.

Indexed as

Adaptation, PhysiologicalAluminumDiacylglycerol O-AcyltransferaseFatty AcidsGenes, PlantPlant ProteinsCell MembraneGene Expression Regulation, PlantGene SilencingLipid PeroxidationPlant RootsPlants, Genetically ModifiedStress, PhysiologicalTranscription FactorsTriglyceridesAluminumDiacylglycerol O-AcyltransferaseFatty AcidsPlant ProteinsTranscription FactorsTriglyceridesaluminum stressATML1Carya cathayensisdiacylglycerol acyltransferasefatty acidsWRKY

Identifiers

PMID42437970
PMCPMC13491255

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.