Molecular characterization of virulence and resistance determinants in clinical Pseudomonas aeruginosa isolates: a cross-sectional analysis of virulence-resistance associations.

Soha Abdallah Moursi, Mohd Saleem, Alharbi Mohammed Salem, Ehab Badran Mohammed Rakha, Ahmed Nawi Alshammari, Rana Aboras, AzharuddinSajid SyedKhaja, Ashfaque Hossain

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Article in Naunyn-Schmiedeberg's archives of pharmacology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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5 · Who and what money

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8 authors.

Soha Abdallah MoursiDepartment of Pathology, College of Medicine, University of Ha'il, Hail, Saudi Arabia. s.moursi@uoh.edu.sa.ORCID https://orcid.org/0000-0002-3922-2247

Mohd SaleemDepartment of Pathology, College of Medicine, University of Ha'il, Hail, Saudi Arabia.

Alharbi Mohammed SalemDepartment of Internal Medicine, College of Medicine, University of Ha'il, Hail, Saudi Arabia.

Ehab Badran Mohammed RakhaLaboratory Department, King Khalid Hospital, Hail, Saudi Arabia.

Ahmed Nawi AlshammariDepartment of Internal Medicine, College of Medicine, University of Ha'il, Hail, Saudi Arabia.

Rana AborasDepartment of Family Medicine, Community Medicine, University of Ha'il, Hail, Saudi Arabia.

AzharuddinSajid SyedKhajaDepartment of Pathology, College of Medicine, University of Ha'il, Hail, Saudi Arabia.

Ashfaque HossainDepartment of Medical Microbiology and Immunology, RAK Medical and Health Science University, Ras Al Khaimah, United Arab Emirates.

Funding

The authors thank all the participants in this research. This research was funded by the Scientific Research Deanship at the University of Ha'il, Saudi Arabia through project number RG-23 201

6 · The paper itself

Abstract

Pseudomonas aeruginosa is a clinically significant opportunistic pathogen characterized by intrinsic and acquired resistance mechanisms coupled with a diverse array of virulence determinants. This study investigated the distribution of P. aeruginosa in various clinical specimens (n = 382), its virulence genes, antimicrobial resistance patterns, and associations with total resistant P. aeruginosa (TRPA) phenotypes. The majority of isolates were recovered from sputum (24.3%) and endotracheal tube samples (22.5%). Virulence gene screening revealed moderate-to-high prevalence of lasB (55.0%), toxA (49.7%), pilA (53.7%), aprA (56.5%), phzS (45.0%), exoS (41.9%), and exoU (38.0%), indicating their widespread involvement in pathogenicity. Antimicrobial susceptibility profiling demonstrated the highest susceptibility to amikacin (56.3%) and gentamicin (52.4%), while resistance to carbapenems (imipenem 52.4%, meropenem 52.9%) and cephalosporins was alarmingly high. Based on resistance classification, 33.5% of isolates were MDR, and 9.7% XDR. Among ceftazidime-resistant isolates (n = 200), 83% harboured ESBL genes, with bla

Indexed as

Antimicrobial resistanceMDRPseudomonas aeruginosaVirulence genesXDR

Identifiers

PMID42437416

DOI10.3389/fmicb.2021.614058

What OpenQuestion holds

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.