Evidence map›Paper›PMID 42436537›Full record

ArticleVeterinary research2026

A new recombinant avian metapneumovirus vaccine candidate provides complete protection against the novel variant infectious bursal disease virus in chickens.

Lingzhai Meng, Yuntong Chen, Mengmeng Yu, Xiaole Qi, Yongzhen Liu, Suyan Wang, Xiaoxiao Xue, Tao Zhang, Wenrui Fan, Zibo Zhang and 7 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Veterinary research, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

17 authors.

Lingzhai MengAvian Immunosuppressive Diseases Division, State Key Laboratory of Animal Disease Control and Prevention, Harbin Veterinary Research Institute, The Chinese Academy of Agricultural Sciences, Harbin, 150069, People's Republic of China.
Yuntong ChenAvian Immunosuppressive Diseases Division, State Key Laboratory of Animal Disease Control and Prevention, Harbin Veterinary Research Institute, The Chinese Academy of Agricultural Sciences, Harbin, 150069, People's Republic of China.
Mengmeng YuAvian Immunosuppressive Diseases Division, State Key Laboratory of Animal Disease Control and Prevention, Harbin Veterinary Research Institute, The Chinese Academy of Agricultural Sciences, Harbin, 150069, People's Republic of China.
Xiaole QiAvian Immunosuppressive Diseases Division, State Key Laboratory of Animal Disease Control and Prevention, Harbin Veterinary Research Institute, The Chinese Academy of Agricultural Sciences, Harbin, 150069, People's Republic of China.
Yongzhen LiuAvian Immunosuppressive Diseases Division, State Key Laboratory of Animal Disease Control and Prevention, Harbin Veterinary Research Institute, The Chinese Academy of Agricultural Sciences, Harbin, 150069, People's Republic of China.
Suyan WangAvian Immunosuppressive Diseases Division, State Key Laboratory of Animal Disease Control and Prevention, Harbin Veterinary Research Institute, The Chinese Academy of Agricultural Sciences, Harbin, 150069, People's Republic of China.
Xiaoxiao XueAvian Immunosuppressive Diseases Division, State Key Laboratory of Animal Disease Control and Prevention, Harbin Veterinary Research Institute, The Chinese Academy of Agricultural Sciences, Harbin, 150069, People's Republic of China.
Tao ZhangAvian Immunosuppressive Diseases Division, State Key Laboratory of Animal Disease Control and Prevention, Harbin Veterinary Research Institute, The Chinese Academy of Agricultural Sciences, Harbin, 150069, People's Republic of China.
Wenrui FanAvian Immunosuppressive Diseases Division, State Key Laboratory of Animal Disease Control and Prevention, Harbin Veterinary Research Institute, The Chinese Academy of Agricultural Sciences, Harbin, 150069, People's Republic of China.
Zibo ZhangAvian Immunosuppressive Diseases Division, State Key Laboratory of Animal Disease Control and Prevention, Harbin Veterinary Research Institute, The Chinese Academy of Agricultural Sciences, Harbin, 150069, People's Republic of China.
Ying WangAvian Immunosuppressive Diseases Division, State Key Laboratory of Animal Disease Control and Prevention, Harbin Veterinary Research Institute, The Chinese Academy of Agricultural Sciences, Harbin, 150069, People's Republic of China.
Ru GuoAvian Immunosuppressive Diseases Division, State Key Laboratory of Animal Disease Control and Prevention, Harbin Veterinary Research Institute, The Chinese Academy of Agricultural Sciences, Harbin, 150069, People's Republic of China.
Mingxue HuAvian Immunosuppressive Diseases Division, State Key Laboratory of Animal Disease Control and Prevention, Harbin Veterinary Research Institute, The Chinese Academy of Agricultural Sciences, Harbin, 150069, People's Republic of China.
Yanping ZhangAvian Immunosuppressive Diseases Division, State Key Laboratory of Animal Disease Control and Prevention, Harbin Veterinary Research Institute, The Chinese Academy of Agricultural Sciences, Harbin, 150069, People's Republic of China.
Yulu DuanAvian Immunosuppressive Diseases Division, State Key Laboratory of Animal Disease Control and Prevention, Harbin Veterinary Research Institute, The Chinese Academy of Agricultural Sciences, Harbin, 150069, People's Republic of China.
Hongyu CuiAvian Immunosuppressive Diseases Division, State Key Laboratory of Animal Disease Control and Prevention, Harbin Veterinary Research Institute, The Chinese Academy of Agricultural Sciences, Harbin, 150069, People's Republic of China.
Yulong GaoAvian Immunosuppressive Diseases Division, State Key Laboratory of Animal Disease Control and Prevention, Harbin Veterinary Research Institute, The Chinese Academy of Agricultural Sciences, Harbin, 150069, People's Republic of China. gaoyulong@caas.cn.

Funding

grants from the China Agriculture Research System CARS-41-G15Innovation Program of Chinese Academy of Agricultural Sciences CAAS-CSLPDCP-202402National Key Research and Development Program of China 2023YFE0106100
6 · The paper itself

Abstract

Infectious bursal disease (IBD) is an acute, highly contagious, and immunosuppressive condition in chickens, caused by the infectious bursal disease virus (IBDV). The recent emergence of novel variant IBDV (nVarIBDV) poses a significant threat to the global poultry industry. However, currently available vaccines provide only limited protection against nVarIBDV strains. In this study, a recombinant avian metapneumovirus subtype B (aMPV/B) expressing the nVarIBDV VP2 gene (rLN16A-nVarVP2) was successfully rescued by inserting the gene between the G and L genes of the attenuated aMPV/B strain LN16-A. Immunofluorescence and Western blotting analyses confirmed stable VP2 expression in vitro. Further evaluation showed that VP2 insertion did not alter the growth kinetics of the parental virus, and the expression remained stable after 20 serial passages. A single immunization with rLN16A-nVarVP2 elicited robust humoral and cellular immune responses in specific pathogen-free chickens, inducing high levels of neutralizing antibodies against both nVarIBDV and aMPV/B, as well as Th1 (IL-2, IFN-γ) and Th2 (IL-4, IL-6) cytokines. Moreover, rLN16A-nVarVP2 conferred complete (100%) protective efficacy against both nVarIBDV and aMPV/B, fully prevented bursal atrophy and clinical signs associated with aMPV/B infection, and markedly decreased bursa viral load (nVarIBDV) and turbinate viral shedding (aMPV/B). Our findings suggest that rLN16A-nVarVP2 represents a promising live attenuated bivalent vaccine candidate for preventing infections caused by nVarIBDV and aMPV/B.

Indexed as

Birnaviridae InfectionsChickensInfectious bursal disease virusMetapneumovirusParamyxoviridae InfectionsPoultry DiseasesViral VaccinesAnimalsSpecific Pathogen-Free OrganismsVaccines, SyntheticVaccines, SyntheticViral Vaccinesavian metapneumovirus subtype BNovel variant infectious bursal disease virusprotective efficacyrLN16A-nVarVP2VP2 gene

Identifiers

PMID42436537
PMCPMC13355354

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.