Evidence map›Paper›PMID 42435220›Full record

ArticleApplied microbiology and biotechnology2026

Targeted genome editing of the non-model cyanobacterium Cyanothece PCC 7425 via CRISPR/Cas12a.

Monis Athar Khan, Anne Durand, Fériel Skouri-Panet, Karim Benzerara, Corinne Cassier-Chauvat, Franck Chauvat, Soufian Ouchane

Abstract read
In one paragraph

Article in Applied microbiology and biotechnology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Monis Athar KhanInstitute for Integrative Biology of the Cell (I2BC), Université Paris-Saclay, CEA, CNRS, Gif-Sur-Yvette, France. monis-athar.khan@i2bc.paris-saclay.fr.ORCID http://orcid.org/0009-0008-4161-5913
Anne DurandInstitute for Integrative Biology of the Cell (I2BC), Université Paris-Saclay, CEA, CNRS, Gif-Sur-Yvette, France.ORCID http://orcid.org/0000-0002-0463-5930
Fériel Skouri-PanetUMR CNRS 7590. Institut de Minéralogie, de Physique Des Matériaux Et de Cosmochimie (IMPMC), Sorbonne Université, Muséum National d'Histoire Naturelle, Paris, France.ORCID http://orcid.org/0000-0002-2623-9058
Karim BenzeraraUMR CNRS 7590. Institut de Minéralogie, de Physique Des Matériaux Et de Cosmochimie (IMPMC), Sorbonne Université, Muséum National d'Histoire Naturelle, Paris, France.ORCID http://orcid.org/0000-0002-0553-0137
Corinne Cassier-ChauvatInstitute for Integrative Biology of the Cell (I2BC), Université Paris-Saclay, CEA, CNRS, Gif-Sur-Yvette, France.ORCID http://orcid.org/0000-0001-6901-5559
Franck ChauvatInstitute for Integrative Biology of the Cell (I2BC), Université Paris-Saclay, CEA, CNRS, Gif-Sur-Yvette, France.ORCID http://orcid.org/0000-0002-9151-7667
Soufian OuchaneInstitute for Integrative Biology of the Cell (I2BC), Université Paris-Saclay, CEA, CNRS, Gif-Sur-Yvette, France. soufian.ouchane@i2bc.paris-saclay.fr.ORCID http://orcid.org/0000-0002-8161-3866

Funding

Agence Nationale de la Recherche ANR-19-CE44-0017
6 · The paper itself

Abstract

Cyanobacteria are diverse photosynthetic microorganisms of great interest for fundamental science and sustainable biotechnological applications. However, their polyploidy makes genetic manipulation challenging and time-consuming. The development of CRISPR/Cas tools has greatly accelerated genome editing and metabolic engineering of some cyanobacterial model species. In this work, we extend the CRISPR/Cas12a system for targeted gene deletion in the non-model cyanobacterium Cyanothece sp. PCC 7425, interesting for its ability to perform intracellular calcium carbonate (CaCO

Indexed as

CRISPR-Cas SystemsCyanotheceGene EditingCalcium CarbonateGene DeletionGene Knockout TechniquesGenome, BacterialHomologous RecombinationCalcium CarbonateCRISPR/Cas12aCyanotheceGene knockoutNon-model cyanobacteria

Identifiers

PMID42435220
PMCPMC13642857

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.