Evidence map›Paper›PMID 42434561›Full record

ArticleFrontiers in microbiology2026

Clinical application of targeted next-generation sequencing in detecting organ/space surgical site infection in patients following biliary-pancreatic surgery.

Yifei Yang, Rong Jiang, Yichen Duan, Zhiang Wang, Yucheng Xu, Neng Tang, Yudong Qiu, Liang Mao, Xu Fu

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Article in Frontiers in microbiology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

9 authors.

Yifei Yang *Department of Pancreatic and Metabolic Surgery, Nanjing Drum Tower Hospital, Affiliated Hospital of Medical School, Nanjing University, Nanjing, China.
Rong Jiang *Jiangsu Health Vocational College, Nanjing, China.
Yichen DuanDepartment of Critical Care Medicine, Jinling Hospital, Affiliated Hospital of Medical School, Nanjing University, Nanjing, China.
Zhiang WangDepartment of Pancreatic and Metabolic Surgery, Nanjing Drum Tower Hospital, Affiliated Hospital of Medical School, Nanjing University, Nanjing, China.
Yucheng XuDepartment of Pancreatic and Metabolic Surgery, Nanjing Drum Tower Hospital, Affiliated Hospital of Medical School, Nanjing University, Nanjing, China.
Neng TangDepartment of Pancreatic and Metabolic Surgery, Nanjing Drum Tower Hospital, Affiliated Hospital of Medical School, Nanjing University, Nanjing, China.
Yudong QiuDepartment of Pancreatic and Metabolic Surgery, Nanjing Drum Tower Hospital, Affiliated Hospital of Medical School, Nanjing University, Nanjing, China.
Liang MaoDepartment of Pancreatic and Metabolic Surgery, Nanjing Drum Tower Hospital, Affiliated Hospital of Medical School, Nanjing University, Nanjing, China.
Xu FuDepartment of Pancreatic and Metabolic Surgery, Nanjing Drum Tower Hospital, Affiliated Hospital of Medical School, Nanjing University, Nanjing, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Background: Organ/space surgical site infection (SSI) is a common and severe complication following biliary-pancreatic surgery. Rapid and accurate diagnosis is crucial for managing organ/space SSI. The clinical application of targeted next-generation sequencing (tNGS) in biliary-pancreatic surgery remains limited. Methods: This retrospective study enrolled 73 consecutive patients who underwent biliary-pancreatic surgery between January 2025 and June 2025, among whom 27 patients were clinically diagnosed with organ/space SSI. Abdominal drainage specimens were collected and analyzed using tNGS and conventional microbiological tests (CMTs). The diagnostic performance, pathogen spectrum, and turnaround time of the two methods were evaluated. Results: The overall pathogen detection rate was higher for tNGS than for CMTs (52.1% vs. 39.7%). tNGS showed higher sensitivity (92.6% vs. 66.7%) and negative predictive value (94.3% vs. 79.5%) than CMTs in organ/space SSI, with a shorter turnaround time (28.1 h vs. 109.1 h). tNGS revealed a broader pathogenic spectrum than CMTs (23 vs. 12 species). Moreover, advanced age (OR = 1.052, 95%CI = 1.005-1.100 Conclusion: tNGS may provide rapid adjunctive microbiological information for early etiological assessment of organ/space SSI after biliary-pancreatic surgery, but it should complement rather than replace CMTs and clinical assessment.

Indexed as

biliary-pancreatic surgeryconventional microbiological testspathogen detectionsurgical site infectiontargeted next-generation sequencing

Identifiers

PMID42434561
PMCPMC13350536

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