Evidence map›Paper›PMID 42434424›Full record

ArticleFrontiers in cellular and infection microbiology2026

A diagnostic performance study of multiplex polymerase chain reaction-based targeted next-generation sequencing for the accurate identification of

Hansheng Wang, Dan Cheng, Xin Qian, Xianru Xia, Biyu Chen, Chenglin Wu, Fang Wang, Sijia Guo, Yujie Gao, Yi Wu and 3 more

Abstract readEvaluation Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Frontiers in cellular and infection microbiology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Hansheng WangDepartment of Pulmonary and Critical Care Medicine, Shiyan Key Laboratory of Major Chronic Respiratory Disease, Taihe Hospital, Hubei University of Medicine, Shiyan, Hubei, China.
Dan ChengDepartment of Blood Transfusion Medicine, Taihe Hospital, Hubei University of Medicine, Shiyan, Hubei, China.
Xin QianDepartment of Pulmonary and Critical Care Medicine, Shiyan Key Laboratory of Major Chronic Respiratory Disease, Taihe Hospital, Hubei University of Medicine, Shiyan, Hubei, China.
Xianru XiaClinical Molecular Diagnosis Center, Taihe Hospital, Hubei University of Medicine, Shiyan, Hubei, China.
Biyu ChenDepartment of Pulmonary and Critical Care Medicine, Shiyan Key Laboratory of Major Chronic Respiratory Disease, Taihe Hospital, Hubei University of Medicine, Shiyan, Hubei, China.
Chenglin WuDepartment of Pulmonary and Critical Care Medicine, Shiyan Key Laboratory of Major Chronic Respiratory Disease, Taihe Hospital, Hubei University of Medicine, Shiyan, Hubei, China.
Fang WangDepartment of Pulmonary and Critical Care Medicine, Shiyan Key Laboratory of Major Chronic Respiratory Disease, Taihe Hospital, Hubei University of Medicine, Shiyan, Hubei, China.
Sijia GuoDepartment of Pulmonary and Critical Care Medicine, Shiyan Key Laboratory of Major Chronic Respiratory Disease, Taihe Hospital, Hubei University of Medicine, Shiyan, Hubei, China.
Yujie GaoDepartment of Pulmonary and Critical Care Medicine, Shiyan Key Laboratory of Major Chronic Respiratory Disease, Taihe Hospital, Hubei University of Medicine, Shiyan, Hubei, China.
Yi WuDepartment of Pulmonary and Critical Care Medicine, Shiyan Key Laboratory of Major Chronic Respiratory Disease, Taihe Hospital, Hubei University of Medicine, Shiyan, Hubei, China.
Tao RenDepartment of Pulmonary and Critical Care Medicine, Shiyan Key Laboratory of Major Chronic Respiratory Disease, Taihe Hospital, Hubei University of Medicine, Shiyan, Hubei, China.
Yijun TangDepartment of Pulmonary and Critical Care Medicine, Shiyan Key Laboratory of Major Chronic Respiratory Disease, Taihe Hospital, Hubei University of Medicine, Shiyan, Hubei, China.
Meifang WangDepartment of Pulmonary and Critical Care Medicine, Shiyan Key Laboratory of Major Chronic Respiratory Disease, Taihe Hospital, Hubei University of Medicine, Shiyan, Hubei, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Objective: To evaluate the diagnostic performance of multiplex PCR-based targeted next-generation sequencing (mp-tNGS) for detecting Methods: This prospective study enrolled 188 patients. BALF samples were tested in parallel by AFB smear, culture, Xpert MTB/RIF, and mp-tNGS. Diagnostic performance was assessed against a composite clinical reference standard; phenotypic drug susceptibility testing (pDST) was additionally compared in a subset of 35 patients. Results: Among the cohort, 116 patients were diagnosed with PTB. The sensitivities of AFB smear, culture, Xpert MTB/RIF, and mp-tNGS were 19.8%, 33.6%, 73.3%, and 83.6%, respectively; specificities were 91.7%, 100%, 98.6%, and 97.2%. mp-tNGS demonstrated the highest diagnostic accuracy (88.8%) and the strongest agreement with the reference standard (κ = 0.868, Conclusion: The mp-tNGS assay provides a superior combination of rapid turnaround time (~12 hours) and high diagnostic accuracy for MTB detection in BALF. Beyond pathogen identification, it offers rapid and preliminary genotypic resistance screening, supporting early suspicion of multidrug resistance, while phenotypic confirmation remains essential. This makes mp-tNGS a highly effective tool for the rapid, accurate diagnosis and preliminary resistance screening of PTB.

Indexed as

Bronchoalveolar Lavage FluidHigh-Throughput Nucleotide SequencingMolecular Diagnostic TechniquesMultiplex Polymerase Chain ReactionMycobacterium tuberculosisTuberculosis, PulmonaryAdultAgedAntitubercular AgentsBacilloscopyDNA-Directed RNA PolymerasesFemaleHumansMaleMicrobial Sensitivity TestsMiddle AgedAntitubercular AgentsDNA-Directed RNA PolymerasesRifampinbronchoalveolar lavage fluid (BALF)drug resistance detectionmycobacterium tuberculosistargeted next-generation sequencing (TNGS)Xpert MTB/RIF

Identifiers

PMID42434424
PMCPMC13350470

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.