Evidence map›Paper›PMID 42433640›Full record

ArticleChina CDC weekly2026

Systematic Evaluation and Application of Single-Nucleotide Polymorphism-Based Genotyping Methods in Varicella-Zoster Virus Molecular Epidemiology Surveillance - China, 2017-2026.

Jinyuan Guo, Zhen Zhu, Naiying Mao, Huiling Wang, Junrui Chen, Hai Li, Lei Cao, Suting Wang, Lixia Fan, Huan Zhang and 10 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in China CDC weekly, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

20 authors.

Jinyuan GuoNational Measles-Rubella Laboratory Network, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China.
Zhen ZhuNational Measles-Rubella Laboratory Network, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China.
Naiying MaoNational Measles-Rubella Laboratory Network, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China.
Huiling WangNational Measles-Rubella Laboratory Network, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China.
Junrui ChenJining Medical University, Jining City, Shandong Province, China.
Hai LiNational Measles-Rubella Laboratory Network, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China.
Lei CaoNational Measles-Rubella Laboratory Network, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China.
Suting WangShandong Center for Disease Control and Prevention, Jinan City, Shandong Province, China.
Lixia FanQinghai Provincial Center for Disease Control and Prevention, Xining City, Qinghai Province, China.
Huan ZhangGuangdong Provincial Center for Disease Control and Prevention, Guangzhou City, Guangdong Province, China.
Libo WangTongliao Center for Disease Control and Prevention, Tongliao City, Inner Mongolia Autonomous Region, China.
Wensi WangLiaoning Provincial Center for Disease Control and Prevention, Shenyang City, Liaoning Province, China.
Xiangpeng ChenLaboratory of Infection and Virology, Beijing Pediatric Research Institute, Beijing Children's Hospital, Beijing, China.
Fangcai LiHunan Provincial Center for Disease Control and Prevention, Changsha City, Hunan Province, China.
Jia HuangXinjiang Uygur Autonomous Region Center for Disease Control and Prevention, Urumqi City, Xinjiang Uygur Autonomous Region, China.
Hongxiong GuoJiangsu Provincial Center for Disease Control and Prevention, Nanjing City, Jiangsu Province, China.
Liqun LiYunnan Center for Disease Control and Prevention, Kunming City, Yunnan Province, China.
Hui ZhangGansu Provincial Center for Disease Control and Prevention, Lanzhou City, Gansu Province, China.
Daxing FengHenan Provincial Centre for Disease Control and Prevention, Zhengzhou City, Henan Province, China.
Yan ZhangNational Measles-Rubella Laboratory Network, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Introduction: In China, no standardized single-nucleotide polymorphism (SNP) scheme exists for varicella-zoster virus (VZV) genotyping. The 5-SNP scheme with two amplicon gene fragments lacks systematic validation. This study aimed to evaluate the accuracy and applicability of this genotyping method. Methods: A total of 280 complete genomes were genotyped using 5 SNPs extracted from ORF22 (four SNPs) and ORF38 (one SNP) fragments. The results were compared with those of the phylogenetic clustering method as a reference. Concordance with reference was used to estimate the accuracy of the SNP scheme. The evaluated SNP scheme was applied to a national VZV surveillance screen containing 549 clinical samples from 17 Chinese provincial-level administrative divisions (2017-2026). Results: A 97.9% concordance was observed between the 5-SNP scheme and phylogenetic clustering methods. The 2.1% discordance was mostly attributed to putative recombination and early circulation of strains from patients with herpes zoster. During the national VZV surveillance screening, 434 samples were amplified, sequenced, and genotyped using a 5-SNP scheme. Of the genotyped samples, 90.3% and 8.8% were identified as clades 2 and 5, respectively, using four ORF22 SNPs, and the remaining 0.9% as clade 4, using ORF38 SNP. All samples showed 100% intragenotypic SNP profile consistency. Conclusion: The unified 5-SNP scheme is accurate and practical for VZV surveillance in China; however, periodic evaluation is required.

Indexed as

genotypingSNPvaricella and zoster virus

Identifiers

PMID42433640
PMCPMC13349669

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.