ArticleLuminescence : the journal of biological and chemical luminescence2026
A Reliable Fluorescence Method of One-Pot Condensation for Sensitive Determination of Epinastine in Aqueous Humor: Greenness, Blueness, and Redness Assessment.
Article in Luminescence : the journal of biological and chemical luminescence, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Epinastine used in treatment and management of allergic conjunctivitis. The development of a simple, sensitive, and cost-effective analytical method for its quantification is essential for pharmaceutical quality control and study. Green (eco-friendly) spectrofluorimetric method for the determination of epinastine in ophthalmic preparations and aqueous humor samples was developed. The method involves converting the cited drug into a fluorescent derivative through a one-pot condensation reaction with fluorescamine, which serves as a detection probe. Fluorescamine is a reagent that reacts specifically with the primary amine moiety of epinastine, forming a highly fluorescent product. In borate buffer (pH 8.5), the reaction yielded a fluorescent product with maximum emission intensity at 483 nm upon excitation at 390 nm. The fluorescence intensity showed a linear response within the concentration ranges of 60-400 ng/mL and 65-350.0 ng/mL for standard epinastine and spiked aqueous humor samples, respectively. The method demonstrated high sensitivity, with limits of detection (LOD) of 19.5 and 20.60 ng/mL and limits of quantification (LOQ) of 59.11 and 62.50 ng/mL for standard epinastine and aqueous humor samples, respectively. The greenness profile of the method was evaluated using four smart metric tools.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.