Evidence map›Paper›PMID 42431582›Full record

ArticleCell stress & chaperones2026

Heat shock transcription factor 1 acts as an endogenous protective mechanism in mechanically stretched alveolar epithelial cells.

Jinqiu Ding, Xinyi Tang, Haoyue Xue, Zuxin Zhou, Haoran Chen, Yao Yan, Yongpeng Xie

Erratum issuedAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell stress & chaperones, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Not yet cited in PubMed.

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0cells of the map it votes in
0citing papers in PubMed
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1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

7 authors.

Jinqiu DingDepartment of Emergency Medicine, The Affiliated Lianyungang Hospital of Xuzhou Medical University, The Lianyungang Clinical College of Nanjing Medical University, The First Affiliated Hospital of Kangda College of Nanjing Medical University, The First People's Hospital of Lianyungang, Lianyungang, Jiangsu 222000, China.
Xinyi TangDepartment of Emergency Medicine, The Affiliated Lianyungang Hospital of Xuzhou Medical University, The Lianyungang Clinical College of Nanjing Medical University, The First Affiliated Hospital of Kangda College of Nanjing Medical University, The First People's Hospital of Lianyungang, Lianyungang, Jiangsu 222000, China; Department of Intensive Care Medicine, Affiliated Hospital of Nantong University, Nantong, Jiangsu 226000, China.
Haoyue XueDepartment of Emergency Medicine, The Affiliated Lianyungang Hospital of Xuzhou Medical University, The Lianyungang Clinical College of Nanjing Medical University, The First Affiliated Hospital of Kangda College of Nanjing Medical University, The First People's Hospital of Lianyungang, Lianyungang, Jiangsu 222000, China.
Zuxin ZhouDepartment of Emergency Medicine, The Affiliated Lianyungang Hospital of Xuzhou Medical University, The Lianyungang Clinical College of Nanjing Medical University, The First Affiliated Hospital of Kangda College of Nanjing Medical University, The First People's Hospital of Lianyungang, Lianyungang, Jiangsu 222000, China.
Haoran ChenDepartment of Emergency Medicine, The Affiliated Lianyungang Hospital of Xuzhou Medical University, The Lianyungang Clinical College of Nanjing Medical University, The First Affiliated Hospital of Kangda College of Nanjing Medical University, The First People's Hospital of Lianyungang, Lianyungang, Jiangsu 222000, China.
Yao YanDepartment of Emergency Medicine, The Affiliated Lianyungang Hospital of Xuzhou Medical University, The Lianyungang Clinical College of Nanjing Medical University, The First Affiliated Hospital of Kangda College of Nanjing Medical University, The First People's Hospital of Lianyungang, Lianyungang, Jiangsu 222000, China.
Yongpeng XieDepartment of Emergency Medicine, The Affiliated Lianyungang Hospital of Xuzhou Medical University, The Lianyungang Clinical College of Nanjing Medical University, The First Affiliated Hospital of Kangda College of Nanjing Medical University, The First People's Hospital of Lianyungang, Lianyungang, Jiangsu 222000, China. Electronic address: xyp8285@njmu.edu.cn.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Mechanical ventilation is a key respiratory support measure for critically ill patients. During improper ventilation, continuous exposure of alveolar epithelial cells (AECs) to abnormal mechanical environment can lead to ventilator-induced lung injury (VILI). Heat shock transcription factor 1 (HSF1) is a stress-responsive transcriptional regulator that orchestrates cytoprotective heat shock protein (HSP) expression in response to diverse stresses, including thermal and oxidative stress, but its role in stretch-induced AEC injury remains unclear. In this study, A549 cells were subjected to biaxial cyclic stretch to model mechanical stress associated with VILI in vitro. Consistent with the established role of YAP as a mechanotransduction regulator activated by cyclic stretch, we observed YAP signaling activation in our biaxial stretch system. Transcriptomic analysis revealed that mechanical stretch markedly induced the expression of HSP genes, and subsequent validation confirmed that stretch activated HSF1 and increased HSF1-dependent HSP expression. Pharmacological inhibition of HSF1 with DTHIB or CRISPR-Cas9-mediated HSF1 knockout attenuated stretch-induced HSP expression, while exacerbating ROS accumulation, cell death, and IL-6 production. Moreover, oxidative-stress-associated HSF1 targets were significantly enriched among stretch-induced genes, antioxidant treatment partially suppressed stretch-induced HSF1 activation, and HSF1 knockout increased oxidative stress after stretch. These findings indicate that HSF1 functions as an endogenous cytoprotective feedback mechanism in mechanically stretched AECs by inducing HSP expression and limiting stretch-induced oxidative damage and inflammatory injury. Activation of the HSF1-HSP axis may therefore represent a potential strategy for mitigating epithelial injury during VILI.

Indexed as

Alveolar Epithelial CellsHeat Shock Transcription FactorsStress, MechanicalA549 CellsHumansOxidative StressReactive Oxygen SpeciesVentilator-Induced Lung InjuryHeat Shock Transcription FactorsHSF1 protein, humanReactive Oxygen SpeciesAlveolar epithelial cellsHeat shock proteinsHeat shock transcription factor 1Mechanical stretchVentilator-induced lung injury

Identifiers

PMID42431582
PMCPMC13427404

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.