In one paragraphArticle in Science advances, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
2 · The registryThe trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
3 · Its place in the literatureWho cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
4 · The recordCorrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
5 · Who and what moneyAuthors and funding
19 authors.
Shirin KappelhoffDepartment of Biology/Chemistry and Center for Cellular Nanoanalytics (CellNanOs), Osnabrück University, Osnabrück, Germany.ORCID 0009-0001-5857-1178 Michael HoltmannspötterDepartment of Biology/Chemistry and Center for Cellular Nanoanalytics (CellNanOs), Osnabrück University, Osnabrück, Germany.ORCID 0000-0003-2396-2627 Stefan L SchaeferDepartment of Theoretical Biophysics, Max Planck Institute of Biophysics, Frankfurt am Main, Germany.ORCID 0000-0001-7942-8701 Eleonora G MargheritisDepartment of Biology/Chemistry and Center for Cellular Nanoanalytics (CellNanOs), Osnabrück University, Osnabrück, Germany.ORCID 0000-0003-0571-3563 Özgün Doga AsikDepartment of Biology/Chemistry and Center for Cellular Nanoanalytics (CellNanOs), Osnabrück University, Osnabrück, Germany.ORCID 0009-0000-6839-725X Nadine GehleDepartment of Biology/Chemistry and Center for Cellular Nanoanalytics (CellNanOs), Osnabrück University, Osnabrück, Germany.ORCID 0009-0004-3984-1058 Hannah VeitDepartment of Biology/Chemistry and Center for Cellular Nanoanalytics (CellNanOs), Osnabrück University, Osnabrück, Germany.ORCID 0009-0006-6553-5235 Rico FranzkochDepartment of Biology/Chemistry and Center for Cellular Nanoanalytics (CellNanOs), Osnabrück University, Osnabrück, Germany.ORCID 0000-0002-8447-518X Sebastian StraussFaculty of Physics and Center for Nanoscience, Ludwig Maximilian University, Munich, Germany.ORCID 0000-0002-4653-1451 Jonatan AlvelidDepartment of Biophysical Imaging, Leibniz Institute of Photonic Technology, Jena, Germany.ORCID 0000-0002-3554-9322 Agnes KoerferDepartment of Biophysical Imaging, Leibniz Institute of Photonic Technology, Jena, Germany.ORCID 0009-0009-7343-2828 Christian EggelingDepartment of Biophysical Imaging, Leibniz Institute of Photonic Technology, Jena, Germany.ORCID 0000-0002-3698-5599 Olympia E PsathakiDepartment of Biology/Chemistry and Center for Cellular Nanoanalytics (CellNanOs), Osnabrück University, Osnabrück, Germany.ORCID 0000-0002-4035-6840 Ralf JungmannFaculty of Physics and Center for Nanoscience, Ludwig Maximilian University, Munich, Germany.ORCID 0000-0003-4607-3312 Rainer KurreDepartment of Biology/Chemistry and Center for Cellular Nanoanalytics (CellNanOs), Osnabrück University, Osnabrück, Germany.ORCID 0000-0002-6872-6567 Gerhard HummerDepartment of Theoretical Biophysics, Max Planck Institute of Biophysics, Frankfurt am Main, Germany.ORCID 0000-0001-7768-746X Jacob PiehlerDepartment of Biology/Chemistry and Center for Cellular Nanoanalytics (CellNanOs), Osnabrück University, Osnabrück, Germany.ORCID 0000-0002-2143-2270 Katia CosentinoDepartment of Biology/Chemistry and Center for Cellular Nanoanalytics (CellNanOs), Osnabrück University, Osnabrück, Germany.ORCID 0000-0002-3796-3500 Funding
No grant is acknowledged in the PubMed record.
6 · The paper itselfAbstract
Gasdermin D (GSDMD) executes pyroptosis by forming membrane pores, yet how these structures assemble and are regulated in cells has remained technically inaccessible. We introduce polymer-supported plasma membranes (PSPMs), which preserve native PM properties while providing cytosolic access for nanoscopic imaging. Combining PSPMs with DNA-PAINT super-resolution microscopy, we visualize human and mouse GSDMD nanostructures directly at the PM of pyroptotic cells and uncover species-specific differences in pore size. Quantitative analyses reveal that GSDMD assembles into heterogeneous macromolecular architectures, including ring-shaped structures, which correlate with PM permeabilization. The palmitoylation-deficient C191A mutant retains minor membrane association but fails to form complete rings, indicating that ring assembly, more than membrane binding, determines pore activity. Last, we identify PI(3,4,5)P
Indexed as
Cell MembraneIntracellular Signaling Peptides and ProteinsNeoplasm ProteinsPhosphate-Binding ProteinsPyroptosisAnimalsGasderminsHumansLipoylationMiceGasderminsGSDMD protein, humanGsdmd protein, mouseIntracellular Signaling Peptides and ProteinsNeoplasm ProteinsPhosphate-Binding Proteins
Identifiers
PMID42430474
PMCPMC13353411
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