Protocol for evaluating microglial phagocytosis in situ in mouse hippocampal slices.

Summer G Paulson, Fritz W Lischka, Jeremy D Rotty

Abstract read

In one paragraph

Article in STAR protocols, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Summer G PaulsonUniformed Services University of the Health Sciences, Department of Biochemistry, Bethesda, MD 20814, USA; The Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD 20817, USA.

Fritz W LischkaUniformed Services University of the Health Sciences, Department of Anatomy, Physiology, and Genetics, Bethesda, MD 20814, USA; Uniformed Services University of the Health Sciences, Biomedical Instrumentation Center, Bethesda, MD 20814, USA; The Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD 20817, USA.

Jeremy D RottyUniformed Services University of the Health Sciences, Department of Biochemistry, Bethesda, MD 20814, USA. Electronic address: jeremy.rotty@usuhs.edu.

Funding

Extracellular matrix sensing in cellular signaling, migration and wound repairR01GM134104 · NIGMS · HENRY M. JACKSON FDN FOR THE ADV MIL/MED · PI ROTTY, JEREMY · 2019 to 2023

$1.6M

NIGMS NIH HHS R01 GM134104

6 · The paper itself

Abstract

Microglia are the primary phagocytes of the central nervous system (CNS). Here, we present a protocol for microinjecting opsonized particles into mouse hippocampal slices followed by two-photon time-lapse microscopy. We describe steps for preparing components, removing the brain, creating coronal slices, and incubating samples. We then detail procedures for analyzing microglial morphology and cell process dynamics with microinjected particles. We also provide several common potential problems encountered during the protocol alongside troubleshooting strategies to counter them. For complete details on the use and execution of this protocol, please refer to Paulson et al.

Indexed as

Cell BiologyMicroscopyNeuroscience

Identifiers

PMID42430244

DOI10.1016/j.xpro.2026.104696

PMCPMC13380708

What OpenQuestion holds

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Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.