Evidence map›Paper›PMID 42430196›Full record

ArticleMicrobiology (Reading, England)2026

Using auxotrophic donor strains to explore pQBR57 plasmid host range among environmental soil bacterial isolates.

Alejandro Marquiegui-Alvaro, Anastasia Kottara, Matthew J N Thomas, Alberto Scarampi, Micaela Chacón, Michael Brockhurst, Neil Dixon

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Microbiology (Reading, England), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Alejandro Marquiegui-AlvaroManchester Institute of Biotechnology (MIB), Department of Chemistry, University of Manchester, Manchester, M1 7DN, UK.
Anastasia KottaraDivision of Evolution, Infection and Genomics, School of Biological Sciences, Michael Smith Building, University of Manchester, Manchester, M13 9PT, UK.
Matthew J N ThomasDivision of Evolution, Infection and Genomics, School of Biological Sciences, Michael Smith Building, University of Manchester, Manchester, M13 9PT, UK.
Alberto ScarampiSchool of Life Sciences, Gibbet Hill Campus, University of Warwick, CV4 7AL, UK.
Micaela ChacónManchester Institute of Biotechnology (MIB), Department of Chemistry, University of Manchester, Manchester, M1 7DN, UK.
Michael BrockhurstDivision of Evolution, Infection and Genomics, School of Biological Sciences, Michael Smith Building, University of Manchester, Manchester, M13 9PT, UK.
Neil DixonManchester Institute of Biotechnology (MIB), Department of Chemistry, University of Manchester, Manchester, M1 7DN, UK.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Plasmid host range (PHR) plays a key role in the spread of ecologically important genes, alongside applications in microbiome engineering and environmental biotechnology. PHR is a complex trait arising from the combination of plasmid, donor and recipient properties. Most studies of PHR use a single donor strain, leaving the role of the donor unexplored and often require genetically tagged recipient strains for counter-selection, which limits the use of non-genetically tractable strains. Here, we applied auxotrophic donor counter-selection in a relatively high-throughput and accessible screening format to characterize PHR across a diverse collection of environmental isolates without the need for recipient engineering. Specifically, we used two auxotrophic donors (

Indexed as

Host SpecificityPlasmidsPseudomonas fluorescensPseudomonas putidaSoil MicrobiologyConjugation, GeneticPhylogenyauxotrophic plasmid donor strainbioremediationhorizontal gene transferplasmid conjugationpQBR57 plasmidPseudomonas

Identifiers

PMID42430196
PMCPMC13367071

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.