Evidence map›Paper›PMID 42430066›Full record

ArticleAnnals of biomedical engineering2026

Murine Abdominal Aortic Aneurysm Intraluminal Thrombus Composition and Structure.

Cortland H Johns, Luke E Schepers, Annabelle M F Foist, Ethan P Foster, Anish Bedi, Niharika Narra, Claudia K Albrecht, Abigail D Cox, Craig J Goergen

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Article in Annals of biomedical engineering, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Cortland H JohnsWeldon School of Biomedical Engineering, Purdue University, 206 S. Martin Jischke Dr., West Lafayette, IN, 47907, USA.
Luke E SchepersWeldon School of Biomedical Engineering, Purdue University, 206 S. Martin Jischke Dr., West Lafayette, IN, 47907, USA.
Annabelle M F FoistWeldon School of Biomedical Engineering, Purdue University, 206 S. Martin Jischke Dr., West Lafayette, IN, 47907, USA.
Ethan P FosterWeldon School of Biomedical Engineering, Purdue University, 206 S. Martin Jischke Dr., West Lafayette, IN, 47907, USA.
Anish BediWeldon School of Biomedical Engineering, Purdue University, 206 S. Martin Jischke Dr., West Lafayette, IN, 47907, USA.
Niharika NarraWeldon School of Biomedical Engineering, Purdue University, 206 S. Martin Jischke Dr., West Lafayette, IN, 47907, USA.
Claudia K AlbrechtWeldon School of Biomedical Engineering, Purdue University, 206 S. Martin Jischke Dr., West Lafayette, IN, 47907, USA.
Abigail D CoxDepartment of Comparative Pathology, Purdue University, West Lafayette, IN, USA.
Craig J GoergenWeldon School of Biomedical Engineering, Purdue University, 206 S. Martin Jischke Dr., West Lafayette, IN, 47907, USA. cgoergen@purdue.edu.ORCID http://orcid.org/0000-0001-8883-7953

Funding

American Heart Association 14SDG18220010American Heart Association 23IAUST1032828
6 · The paper itself

Abstract

purposeMany abdominal aortic aneurysm (AAA) patients develop intraluminal thrombus (ILT), but the role of ILT in AAA progression and rupture is poorly understood.

methodsTo evaluate ILT in AAAs, we induced AAAs in C57Bl6/J mice via surgical application of topical elastase to the abdominal aorta below the renal arteries and administration of β-aminopropionitrile (BAPN) in drinking water. Ultrasound images of these AAAs were collected longitudinally over a period of 56 days to evaluate AAA growth over time. We semi-quantitatively assessed elastin degradation and inflammation from Movat's pentachrome and H&E-stained samples, respectively. Additionally, we assessed extracellular matrix composition and elastin quality through Movat's pentachrome stained samples.

resultsMice subjected to elastase + BAPN exhibited continuous and significant AAA growth over time. Mice with ILT had more significant expansion over the length of the study (p < 0.05). From histology, ILT samples showed more elastin damage, greater collagen and proteoglycan accumulation, and greater inflammation than aneurysms without ILT. From scanning electron microscopy, we confirmed the presence of fibrin sheets and abnormally shaped red blood cells (polyhedrocytes) within ILT. We also observed that tissue samples with greater inflammation were correlated with more elastin damage. Increased aortic expansion and wall damage may create hemodynamic conditions conducive to initiation of ILT deposition: endothelial damage and reduced blood flow.

conclusionUnderstanding the relationship between ILT formation, aortic wall degradation, and inflammation could help refine clinical strategies for monitoring and treating AAAs. These data suggest that work focused on modulating inflammation and ILT formation could offer promising pathways for non-surgical intervention in AAA treatment.

Indexed as

Abdominal aortic aneurysmIntraluminal thrombus formationScanning electron microscopyUltrasound

Identifiers

PMID42430066

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.