Evidence map›Paper›PMID 42429871›Full record

ArticleWorld journal of microbiology & biotechnology2026

Towards a reduced-background cell factory: disruption of a PKS-NRPS hybrid gene (AlepA) abolishing the production of 2-pyridones in Aspergillus oryzae.

Ruya Yin, Ziqi Zhai, Mengwei Zhang, Yifei Qin, Jianing Wang, Xingrui Liang, Yanxue Peng, Xuan Zhang, Dan Xu, Ligang Zhou and 1 more

Abstract read
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In one paragraph

Article in World journal of microbiology & biotechnology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

11 authors.

Ruya YinState Key Laboratory of Agricultural and Forestry Biosecurity, MARA Key Lab of Pest Monitoring and Green Management, College of Plant Protection, China Agricultural University, Beijing, 100193, China.
Ziqi ZhaiState Key Laboratory of Agricultural and Forestry Biosecurity, MARA Key Lab of Pest Monitoring and Green Management, College of Plant Protection, China Agricultural University, Beijing, 100193, China.
Mengwei ZhangState Key Laboratory of Agricultural and Forestry Biosecurity, MARA Key Lab of Pest Monitoring and Green Management, College of Plant Protection, China Agricultural University, Beijing, 100193, China.
Yifei QinState Key Laboratory of Agricultural and Forestry Biosecurity, MARA Key Lab of Pest Monitoring and Green Management, College of Plant Protection, China Agricultural University, Beijing, 100193, China.
Jianing WangState Key Laboratory of Agricultural and Forestry Biosecurity, MARA Key Lab of Pest Monitoring and Green Management, College of Plant Protection, China Agricultural University, Beijing, 100193, China.
Xingrui LiangState Key Laboratory of Agricultural and Forestry Biosecurity, MARA Key Lab of Pest Monitoring and Green Management, College of Plant Protection, China Agricultural University, Beijing, 100193, China.
Yanxue PengState Key Laboratory of Agricultural and Forestry Biosecurity, MARA Key Lab of Pest Monitoring and Green Management, College of Plant Protection, China Agricultural University, Beijing, 100193, China.
Xuan ZhangState Key Laboratory of Agricultural and Forestry Biosecurity, MARA Key Lab of Pest Monitoring and Green Management, College of Plant Protection, China Agricultural University, Beijing, 100193, China.
Dan XuState Key Laboratory of Agricultural and Forestry Biosecurity, MARA Key Lab of Pest Monitoring and Green Management, College of Plant Protection, China Agricultural University, Beijing, 100193, China.
Ligang ZhouState Key Laboratory of Agricultural and Forestry Biosecurity, MARA Key Lab of Pest Monitoring and Green Management, College of Plant Protection, China Agricultural University, Beijing, 100193, China.
Daowan LaiState Key Laboratory of Agricultural and Forestry Biosecurity, MARA Key Lab of Pest Monitoring and Green Management, College of Plant Protection, China Agricultural University, Beijing, 100193, China. dwlai@cau.edu.cn.ORCID https://orcid.org/0000-0001-7984-5855

Funding

National Key Research and Development Program of China 2023YFD1401000
6 · The paper itself

Abstract

Aspergillus oryzae NSAR1 is a versatile and commonly used heterologous expression host. Typically, this host is used to express exogenous biosynthetic genes for secondary metabolites (SMs). However, the metabolite profile of A. oryzae itself is not clearly understood. In our course to enrich minor metabolites from heterologous expression using in situ macroporous resins, two metabolites were found to be significantly enhanced in A. oryzae itself. These metabolites were isolated, purified, and identified as 2-pyridones, including leporin B (1), and protoleporin A (2), as the major compounds, along with a minor of leporin C (3). The structural elucidation was performed by detailed NMR and HRMS analysis, and by comparison with the literature. This was the first observation of leporin-type 2-pyridones from A. oryzae, among which compound 2 was a new compound. Leporin B was found to display inhibition against Phytophthora sojae. A putative biosynthetic gene cluster (BGC, Aolep) harboring a backbone PKS-NRPS encoding gene (AolepA) was identified for these metabolites through genome mining. Subsequently, AolepA was knocked out using homologous recombination via conventional gene knockout (KO) and CRISPR-Cas9 mediated gene-editing system. Successful disruption of AolepA was achieved using both strategies leading to abolish the production of 2-pyridones completely. The KO mutants showed a similar phenotype as compared to the parent strain, regarding the growth and sporulation. The utility of these mutants as a new chassis was demonstrated by heterologous expression of a 1,3,6,8-tetrahydroxynaphthalene synthase. Hence, a genetic dereplication version of A. oryzae (NSAR1∆L) was generated, featuring a reduced background in endogenous SM production, which should facilitate the study of exogenous genes in SM biosynthesis by alleviating undesired metabolic burden, simplifying the detection and purification of heterologous compounds.

Indexed as

Aspergillus oryzaePeptide SynthasesPolyketide SynthasesPyridonesBiosynthetic PathwaysFungal ProteinsMultigene FamilySecondary Metabolism2-hydroxypyridineFungal Proteinsnon-ribosomal peptide synthasePeptide SynthasesPolyketide SynthasesPyridonesAspergillus oryzaeBiosynthetic gene clusterCell factoryCRISPR/Cas9Gene disruptionheterologous expressionLeporinSecondary metabolites

Identifiers

PMID42429871

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.