Evidence map›Paper›PMID 42429766›Full record

ArticleApplied and environmental microbiology2026

Rational selection of ketopantoate hydroxymethyltransferase with low product feedback inhibition based on binding free energy calculation analysis.

Ken Zheng, Ai-Ping Pang, Fang-Ying Zhu, Yu-Hui Liu, Xiao-Jian Zhang, Qing Yang, Xiang-Yang Li, Jun-Ping Zhou, Bo Zhang, Zhi-Qiang Liu and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Applied and environmental microbiology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Ken ZhengState Key Laboratory of Green Chemical Synthesis and Conversion, Zhejiang University of Technology, Hangzhou, Zhejiang, China.ORCID 0009-0001-6298-3079
Ai-Ping PangState Key Laboratory of Green Chemical Synthesis and Conversion, Zhejiang University of Technology, Hangzhou, Zhejiang, China.
Fang-Ying ZhuState Key Laboratory of Green Chemical Synthesis and Conversion, Zhejiang University of Technology, Hangzhou, Zhejiang, China.
Yu-Hui LiuState Key Laboratory of Green Chemical Synthesis and Conversion, Zhejiang University of Technology, Hangzhou, Zhejiang, China.
Xiao-Jian ZhangState Key Laboratory of Green Chemical Synthesis and Conversion, Zhejiang University of Technology, Hangzhou, Zhejiang, China.
Qing YangState Key Laboratory of Green Chemical Synthesis and Conversion, Zhejiang University of Technology, Hangzhou, Zhejiang, China.
Xiang-Yang LiState Key Laboratory of Green Chemical Synthesis and Conversion, Zhejiang University of Technology, Hangzhou, Zhejiang, China.
Jun-Ping ZhouState Key Laboratory of Green Chemical Synthesis and Conversion, Zhejiang University of Technology, Hangzhou, Zhejiang, China.
Bo ZhangState Key Laboratory of Green Chemical Synthesis and Conversion, Zhejiang University of Technology, Hangzhou, Zhejiang, China.
Zhi-Qiang LiuState Key Laboratory of Green Chemical Synthesis and Conversion, Zhejiang University of Technology, Hangzhou, Zhejiang, China.ORCID 0000-0003-3259-6796
Yu-Guo ZhengState Key Laboratory of Green Chemical Synthesis and Conversion, Zhejiang University of Technology, Hangzhou, Zhejiang, China.

Funding

Key Research and Development Program of Zhejiang Province (Key R&D plan of Zhejiang Province) 2021C03091National Key Research and Development Program of China 2022YFA0911800
6 · The paper itself

Abstract

The microbial production of pantothenic acid (d-PA) is critically limited by feedback inhibition and low activity of the key enzyme ketopantoate hydroxymethyltransferase (KPHMT). To overcome this, rational enzyme mining based on computational prediction was established. Following sequence conservation analysis, molecular dynamics simulations, and binding free energy (ΔG) calculations, five representative native KPHMT enzymes were selected for experimental validation:

Indexed as

Bacterial ProteinsBacillus subtilisCorynebacterium glutamicumEscherichia coliFeedback, PhysiologicalMolecular Dynamics SimulationThermodynamicsBacterial Proteinsd-PAfeedback inhibitiongene miningketopantoate hydroxymethyltransferasepantoate

Identifiers

PMID42429766
PMCPMC13488298

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.