Evidence map›Paper›PMID 42429608›Full record

ArticleAdvanced science (Weinheim, Baden-Wurttemberg, Germany)2026

Design and Engineering of an Artificial Bifunctional N-Deacetylase/N-Sulfotransferase for the Biosynthesis of N-Sulfated Heparosan.

Xintong Xi, Ruirui Xu, Daoan Wang, Guobin Yin, Xinjing Wang, Haiqin Chen, Guocheng Du, Jian Chen, Zhen Kang

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Xintong XiThe Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Jiangnan University, Wuxi, China.
Ruirui XuThe Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Jiangnan University, Wuxi, China.
Daoan WangThe Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Jiangnan University, Wuxi, China.
Guobin YinThe Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Jiangnan University, Wuxi, China.
Xinjing WangThe Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Jiangnan University, Wuxi, China.
Haiqin ChenSchool of Food Science and Technology, Jiangnan University, Wuxi, China.
Guocheng DuThe Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Jiangnan University, Wuxi, China.ORCID https://orcid.org/0000-0002-3269-3476
Jian ChenThe Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Jiangnan University, Wuxi, China.
Zhen KangThe Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Jiangnan University, Wuxi, China.ORCID https://orcid.org/0000-0003-1479-3075

Funding

Fundamental Research Funds for the Central Universities JUSRP622003Guangdong S&T Program 2024B1111160002Jiangsu Basic Research Center for Synthetic Biology BK20233003Jiangsu Provincial Major Science and Technology Special Project BG2025044National Key Research and Development Program of China 2024YFF1106300National Natural Science Foundation of China 32370066National Natural Science Foundation of China 32501356National Natural Science Foundation of China U24A20368Postdoctoral Fellowship Program of CPSF GZC20251638
6 · The paper itself

Abstract

Heparin is a widely used anticoagulant, while its traditional animal-derived production faces significant challenges in safety and scalability. The active expression of the initiating-step bifunctional enzyme N-deacetylase/N-sulfotransferase (NDST) in bacteria remains a key bottleneck in heparin biosynthesis. Here, we firstly mined and characterized diverse polysaccharide N-deacetylases (NDases) in Escherichia coli and revealed several NDases that are active toward the deacetylation of heparosan. An artificial ND

Indexed as

artificial bifunctional enzymeEscherichia coliheparinN‐deacetylase/N‐sulfotransferaseN‐sulfated heparosanpolysaccharide

Identifiers

PMID42429608
PMCPMC13353177

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.