ReviewJournal of separation science2026
Modern Optimization Strategies in High-Performance Liquid Chromatography Analysis.
Review in Journal of separation science, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Authors and funding
2 authors.
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Abstract
Optimization is a critical step in chromatographic method development. High-performance liquid chromatography (HPLC) remains the method of choice for pharmaceutical and biological analysis; however, traditional method development has relied on trial-and-error strategies. These approaches are often laborious, time-consuming, and highly dependent on analyst experience. To address these limitations, structured and data-driven optimization strategies such as Design of Experiments and Analytical Quality by Design have been introduced to improve efficiency and reproducibility. Nevertheless, these approaches often assume ideal system behavior and may not fully capture real-world chromatographic complexity, including peak tailing, matrix interference, and atypical biomolecule retention, particularly in advanced HPLC modes. In this context, artificial intelligence (AI) has emerged as a powerful tool to address nonlinear, multidimensional chromatographic systems. This review provides a comprehensive overview of classical, statistical, and AI-driven optimization strategies in HPLC, highlighting their advantages, limitations, and practical applications. It also proposes a structured framework for selecting appropriate optimization approaches based on system complexity and data availability.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.