ArticlemicroPublication biology2026
A simplified and high-yield purification method for recombinant RNase R.
Article in microPublication biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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2 authors.
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Abstract
RNase R is a 3'→5' exoribonuclease that selectively degrades linear RNAs. As such, RNase R has become a key reagent for circRNA production and analysis. This is particularly true for the in vitro production of circRNA research tools and therapeutic candidates. Unfortunately, the high cost of commercial RNase R is restrictive. We report an accessible, cost-effective method to purify high-quality recombinant RNase R from E. coli using single-step Ni NTA chromatography on entry level FPLC systems. By achieving complete linear RNA digestion while maintaining circRNA, the resulting enzyme matches performance attributes of commercially available RNase R.
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