ArticlebioRxiv : the preprint server for biology2026
Genetic Code Expansion for Site-Specific Encoding of a Switchable, Intrinsic Fluorophore-Quencher Pair to Monitor Protein Dynamics.
Priyanda Giri, Yarra Venkatesh, Moriah H Mathis, Christina M Hurley, Chloe M Jones, Olivier Ndogo Eteme, Zachary M Hostetler, Richard B Cooley, Rahul M Kohli, Ryan A Mehl and 1 more
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In one paragraphArticle in bioRxiv : the preprint server for biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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5 · Who and what moneyAuthors and funding
11 authors.
Priyanda GiriDepartment of Chemistry, School of Arts and Sciences, University of Pennsylvania, 231 South 34th Street, Philadelphia, PA 19104, USA.
Yarra VenkateshDepartment of Chemistry, School of Arts and Sciences, University of Pennsylvania, 231 South 34th Street, Philadelphia, PA 19104, USA.ORCID 0000-0002-4478-1553 Moriah H MathisDepartment of Biochemistry & Biophysics, Oregon State University, Corvallis, OR 97331, USA.ORCID 0000-0001-5182-0234 Christina M HurleyGraduate Group in Biochemistry and Molecular Biophysics, Perelman School of Medicine, University of Pennsylvania, 3620 Hamilton Walk, Philadelphia, PA 19104, USA.ORCID 0000-0002-8821-7233 Chloe M JonesGraduate Group in Biochemistry and Molecular Biophysics, Perelman School of Medicine, University of Pennsylvania, 3620 Hamilton Walk, Philadelphia, PA 19104, USA.ORCID 0000-0002-7131-685X Olivier Ndogo EtemeDepartment of Chemistry, School of Arts and Sciences, University of Pennsylvania, 231 South 34th Street, Philadelphia, PA 19104, USA.ORCID 0009-0008-2900-2487 Zachary M HostetlerDepartment of Medicine, Perelman School of Medicine, University of Pennsylvania, 3400 Civic Center Boulevard, Philadelphia, PA 19104, USA.ORCID 0000-0002-2830-8870 Richard B CooleyDepartment of Biochemistry & Biophysics, Oregon State University, Corvallis, OR 97331, USA.ORCID 0000-0003-3928-2757 Rahul M KohliDepartment of Medicine, Perelman School of Medicine, University of Pennsylvania, 3400 Civic Center Boulevard, Philadelphia, PA 19104, USA.ORCID 0000-0002-7689-5678 Ryan A MehlDepartment of Biochemistry & Biophysics, Oregon State University, Corvallis, OR 97331, USA.ORCID 0000-0003-2932-4941 E James PeterssonDepartment of Chemistry, School of Arts and Sciences, University of Pennsylvania, 231 South 34th Street, Philadelphia, PA 19104, USA.ORCID 0000-0003-3854-9210 Funding
The GCE4All Center: Unleashing the Potential of Genetic Code Expansion for Biomedical ResearchRM1GM144227 · NIGMS · OREGON STATE UNIVERSITY · PI RYAN A MEHL · 2022 to 2026
$6.2MStructural biology and molecular biophysics training programT32GM132039 · NIGMS · UNIVERSITY OF PENNSYLVANIA · PI Ben E. Black, Elizabeth Rhoades · 2019 to 2026
$3.3MPredoctoral Training at the Chemistry-Biology InterfaceT32GM133398 · NIGMS · UNIVERSITY OF PENNSYLVANIA · PI Ronen Marmorstein, Ernest James Petersson · 2020 to 2026
$2.4MCombined Adult and Pediatric Infectious Disease Postdoctoral Training GrantT32AI118684 · NIAID · UNIVERSITY OF PENNSYLVANIA · PI Rahul Manu Kohli, Audrey Ragan Odom John · 2016 to 2026
$2.3MThe Molecular Basis for the Bacterial SOS SignalR01GM127593 · NIGMS · UNIVERSITY OF PENNSYLVANIA · PI KOHLI, RAHUL MANU, PETERSSON, ERNEST JAMES · 2018 to 2021
$1.2MBruker RapifleX MALDI TOF/TOF Mass SpectrometerS10OD030460 · OD · UNIVERSITY OF PENNSYLVANIA · PI PETERSSON, ERNEST JAMES · 2021 to 2021
$881kTime-Of-Flight Instrument with Variable Ionization SourcesS10RR023444 · NCRR · UNIVERSITY OF PENNSYLVANIA · PI DMOCHOWSKI, IVAN JULIAN · 2007 to 2007
$308kNCRR NIH HHS S10 RR023444NIAID NIH HHS T32 AI118684NIGMS NIH HHS R01 GM127593NIGMS NIH HHS RM1 GM144227NIGMS NIH HHS T32 GM132039NIGMS NIH HHS T32 GM133398NIH HHS S10 OD030460
6 · The paper itselfAbstract
Precisely modifying proteins at multiple sites in their native, folded structures offers unique opportunities to answer molecular and cellular-level biological questions. Here, we present a genetic code expansion strategy for site-specific integration of a fluorophore-quencher pair comprising two non-canonical amino acids-acridonylalanine (Acd) and methyltetrazinyl phenylalanine (Tet)- into a protein expressed in
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PMID42427621
PMCPMC13345037
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