Evidence map›Paper›PMID 42426988›Full record

ArticleMicrobial biotechnology2026

Genetic Toolbox Expansion Enables Constitutively Fluorescent Lacticaseibacillus rhamnosus for Functional Microbiome Research.

Ilke Van Tente, Marc Blanch-Asensio, Tom Eilers, Dieter Vandenheuvel, Sarah Lebeer, Shrikrishnan Sankaran, Irina Spacova

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Microbial biotechnology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Ilke Van TenteLaboratory of Applied Microbiology and Biotechnology, Department of Bioscience Engineering, University of Antwerp, Antwerp, Belgium.ORCID https://orcid.org/0000-0002-9287-3735
Marc Blanch-AsensioBioprogrammable Materials, INM-Leibniz Institute for New Materials, Saarbrücken, Germany.ORCID https://orcid.org/0009-0005-8029-601X
Tom EilersLaboratory of Applied Microbiology and Biotechnology, Department of Bioscience Engineering, University of Antwerp, Antwerp, Belgium.ORCID https://orcid.org/0000-0002-7509-2902
Dieter VandenheuvelLaboratory of Applied Microbiology and Biotechnology, Department of Bioscience Engineering, University of Antwerp, Antwerp, Belgium.ORCID https://orcid.org/0000-0002-4398-1916
Sarah LebeerLaboratory of Applied Microbiology and Biotechnology, Department of Bioscience Engineering, University of Antwerp, Antwerp, Belgium.ORCID https://orcid.org/0000-0002-9400-6918
Shrikrishnan SankaranBioprogrammable Materials, INM-Leibniz Institute for New Materials, Saarbrücken, Germany.ORCID https://orcid.org/0000-0001-6251-585X
Irina SpacovaLaboratory of Applied Microbiology and Biotechnology, Department of Bioscience Engineering, University of Antwerp, Antwerp, Belgium.ORCID https://orcid.org/0000-0003-0562-7489

Funding

Deutsche Forschungsgemeinschaft 455063657European Research Council 852600Fonds Wetenschappelijk Onderzoek (FWO SBO DeVeniR) S006424NInteruniversitair Bijzonder Onderzoeksfonds (iBOF) - Inter-Universitary Special Research Fund of Flanders (POSSIBL project) iBOF/21/092Leibniz-GemeinschaftUniversiteit Antwerpen
6 · The paper itself

Abstract

Lacticaseibacillus rhamnosus strains are widely recognized for their probiotic potential and relevance in urogenital and gut health. However, their genetic tractability and genetic tools remain limited, hindering functional microbiome research and synthetic biology applications. In this study, we expanded the genetic toolbox for the widely used probiotic strains, L. rhamnosus GR-1 and L. rhamnosus GG, by implementing direct plasmid cloning and testing of a set of genetic elements earlier validated in Lactiplantibacillus plantarum. Among five constitutive promoters (P

Indexed as

Genetics, MicrobialLacticaseibacillus rhamnosusMicrobiotaGenes, ReporterLuminescent ProteinsPlasmidsPromoter Regions, GeneticLuminescent Proteins

Identifiers

PMID42426988
PMCPMC13350346

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.