Evidence map›Paper›PMID 42426933›Full record

ArticleActa neuropathologica communications2026

Laser microdissection-based multi-omics integration unveils the pathological atlas and tumor differentiation network of adamantinomatous craniopharyngioma.

Bowen Wu, Chunming Xu, Chenxing Yang, Shenhao Xie, Suping Duan, Jiye Ye, Jinshi Zhang, Shaoyang Li, Furong Liang, Zhicheng Ye and 11 more

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Article in Acta neuropathologica communications, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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5 · Who and what money

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21 authors.

Bowen Wu *Department of Neurosurgery, Jiangxi Key Laboratory of Neurological Diseases, Jiangxi Medical College, the First Affiliated Hospital, Nanchang University, 17 Yong Wai Zheng Street, Nanchang, 330006, China.
Chunming Xu *Department of Neurosurgery, Jiangxi Key Laboratory of Neurological Diseases, Jiangxi Medical College, the First Affiliated Hospital, Nanchang University, 17 Yong Wai Zheng Street, Nanchang, 330006, China.
Chenxing Yang *Department of Neurosurgery, Jiangxi Key Laboratory of Neurological Diseases, Jiangxi Medical College, the First Affiliated Hospital, Nanchang University, 17 Yong Wai Zheng Street, Nanchang, 330006, China.
Shenhao Xie *Department of Neurosurgery, Jiangxi Key Laboratory of Neurological Diseases, Jiangxi Medical College, the First Affiliated Hospital, Nanchang University, 17 Yong Wai Zheng Street, Nanchang, 330006, China.
Suping DuanDepartment of Neurology, Jiangxi Medical College, the First Affiliated Hospital, Nanchang University, Nanchang, 330006, China.
Jiye YeDepartment of Neurosurgery, Hunan University of Medicine General Hospital, Huaihua, 418000, China.
Jinshi ZhangDepartment of Neurosurgery, Ganzhou Hospital-Nanfang Hospital, Southern Medical University (Ganzhou People's Hospital), Ganzhou, 341000, China.
Shaoyang LiSchool of public health, Jiangxi medical college, Nanchang University, Nanchang, 330006, China.
Furong LiangDepartment of Neurosurgery, Jiangxi Key Laboratory of Neurological Diseases, Jiangxi Medical College, the First Affiliated Hospital, Nanchang University, 17 Yong Wai Zheng Street, Nanchang, 330006, China.
Zhicheng YeDepartment of Neurosurgery, Jiangxi Key Laboratory of Neurological Diseases, Jiangxi Medical College, the First Affiliated Hospital, Nanchang University, 17 Yong Wai Zheng Street, Nanchang, 330006, China.
Hai LuoDepartment of Neurosurgery, Jiangxi Key Laboratory of Neurological Diseases, Jiangxi Medical College, the First Affiliated Hospital, Nanchang University, 17 Yong Wai Zheng Street, Nanchang, 330006, China.
Rilege SuDepartment of Neurosurgery, Jiangxi Key Laboratory of Neurological Diseases, Jiangxi Medical College, the First Affiliated Hospital, Nanchang University, 17 Yong Wai Zheng Street, Nanchang, 330006, China.
Laisheng PanDepartment of Neurosurgery, Jiangxi Key Laboratory of Neurological Diseases, Jiangxi Medical College, the First Affiliated Hospital, Nanchang University, 17 Yong Wai Zheng Street, Nanchang, 330006, China.
Zhizheng LiuDepartment of Neurosurgery, Jiangxi Key Laboratory of Neurological Diseases, Jiangxi Medical College, the First Affiliated Hospital, Nanchang University, 17 Yong Wai Zheng Street, Nanchang, 330006, China.
Shizhou XingDepartment of Neurosurgery, Jiangxi Key Laboratory of Neurological Diseases, Jiangxi Medical College, the First Affiliated Hospital, Nanchang University, 17 Yong Wai Zheng Street, Nanchang, 330006, China.
Tianming DongDepartment of Neurosurgery, Jiangxi Key Laboratory of Neurological Diseases, Jiangxi Medical College, the First Affiliated Hospital, Nanchang University, 17 Yong Wai Zheng Street, Nanchang, 330006, China.
Yiguang ChenDepartment of Neurosurgery/Neuro-oncology, State Key Laboratory of Oncology in South China, Guangdong Provincial Clinical Research Center for Cancer, SunYat-sen University Cancer Center, Guangzhou, 510060, China.
Like ChenDepartment of Neurosurgery, Jiangxi Key Laboratory of Neurological Diseases, Jiangxi Medical College, the First Affiliated Hospital, Nanchang University, 17 Yong Wai Zheng Street, Nanchang, 330006, China.
Suyue ZhengDepartment of Neurosurgery, Jiangxi Key Laboratory of Neurological Diseases, Jiangxi Medical College, the First Affiliated Hospital, Nanchang University, 17 Yong Wai Zheng Street, Nanchang, 330006, China.
Jie WuDepartment of Neurosurgery, Jiangxi Key Laboratory of Neurological Diseases, Jiangxi Medical College, the First Affiliated Hospital, Nanchang University, 17 Yong Wai Zheng Street, Nanchang, 330006, China. ndyfy10128@ncu.edu.cn.
Tao HongDepartment of Neurosurgery, Jiangxi Key Laboratory of Neurological Diseases, Jiangxi Medical College, the First Affiliated Hospital, Nanchang University, 17 Yong Wai Zheng Street, Nanchang, 330006, China. ndyfy00567@ncu.edu.cn.

Funding

Ganpo555 Engineering Excellence of the Jiangxi Science and Technology Department Grant/Award Number: 2013National Natural Science Foundation of China Grant/Award Number: 82360611the Natural Science Foundation of Jiangxi Province grant no. 20242BAB25529the Science and Technology Plan Project of Health Commission of Jiangxi Province 2023ZD002The Youth Talent Cultivation Project of First Affiliated Hospital of Nanchang University YFYPY2023106
6 · The paper itself

Abstract

Although adamantinomatous craniopharyngioma (ACP) is a histologically benign intracranial tumor, its invasive growth pattern, characterized by finger-like protrusions, leads to a poor prognosis. In-depth analysis of the heterogeneity of the pathological structure of ACP and the development and evolution of tumor cells is crucial for expanding precision treatment strategies. However, a detailed understanding of the complex pathological structural characteristics of ACP is lacking. Here, we used laser microdissection to obtain 40 samples from 6 different pathological structures for Smart-Seq2 sequencing and conducted in-depth analysis of 6 single-nucleus RNA sequencing samples and 4 spatial transcriptome samples. We also performed comprehensive validation using 3 external single-cell databases. Through histopathological observation, multiomic analysis, and immunofluorescence staining, we resolved the single-cell expression profiles of two distinct functional subpopulations of the whorl-like cell clusters, providing insights into their potential biological roles in tumor progression. Additionally, we discovered the epithelial-mesenchymal transition (EMT) or partial EMT development direction of the stellate reticulum and different cell fates related to terminal keratinization. Moreover, we reported the presence of previously neglected cyst-wall epithelium and the constitutive epithelium within ACP. The latter was found to possess basal cell characteristics and act as a potential progenitor cell pool for tumor maintenance and evolution. Through pseudotime analysis combined with co-expression network analysis, we constructed a differentiation regulatory network based on the constitutive epithelium. Our research results complement existing knowledge and provide a comprehensive analysis of the pathological features of ACP, providing new concepts and targets for precision targeted therapy.

Indexed as

CraniopharyngiomaPituitary NeoplasmsCell DifferentiationEpithelial-Mesenchymal TransitionFemaleHumansLaser Capture MicrodissectionMaleMultiomicsAdamantinomatous craniopharyngiomaHeterogeneityLaser microdissectionMultiomics integrationPathologyTumor differentiation

Identifiers

PMID42426933
PMCPMC13640193

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