Evidence map›Paper›PMID 42426472›Full record

ArticleTAG. Theoretical and applied genetics. Theoretische und angewandte Genetik2026

The mutation of pseudouridine synthase gene BrTRUD1 resulted in glossy leaf in Chinese cabbage.

Ningqi Zhao, Gengxing Song, Xinyuan Li, Chang Liu, Jiaqi Zou, Guoliang Yang, Zhiyong Liu, Hui Feng

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Article in TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

8 authors.

Ningqi Zhao *College of Horticulture, Shenyang Agricultural University, Shenyang, 110866, China.
Gengxing Song *College of Horticulture Science and Engineering, Shandong Agricultural University, Taian, 271001, China.
Xinyuan LiCollege of Horticulture, Shenyang Agricultural University, Shenyang, 110866, China.
Chang LiuCollege of Horticulture, Shenyang Agricultural University, Shenyang, 110866, China.
Jiaqi ZouCollege of Horticulture and Landscape Architecture, Southwest University, Chongqing, 400715, China.
Guoliang YangCollege of Horticulture, Shenyang Agricultural University, Shenyang, 110866, China.
Zhiyong Liu *College of Horticulture, Shenyang Agricultural University, Shenyang, 110866, China.
Hui Feng *College of Horticulture, Shenyang Agricultural University, Shenyang, 110866, China. fenghuiaaa@syau.edu.cn.ORCID http://orcid.org/0000-0002-6345-6633

Funding

The Earmarked Fund for CARS-23 and Project of Education Department of Liaoning Province LJ232510157001The National Key Research and Development Program of China 2022YFD12005
6 · The paper itself

Abstract

key messageBrTRUD1 encoding pseudouridine synthase was responsible for the glossy leaf in Chinese cabbage, and its function was verified via Arabidopsis transformation. Leaf glossiness is a crucial commercial trait for assessing the market value of Chinese cabbage. We isolated a glossy leaf mutant wdm21 from an EMS-mutagenized population of a DH line 'FT.' Genetic analysis revealed that the mutant trait was controlled by a single recessive nuclear gene. MutMap sequencing and KASP genotyping assays identified BraA03g032280.3.5C as the candidate gene. BraA03g032280.3.5C is an ortholog of Arabidopsis AtTRUD1 encoding pseudouridine synthase. A C-T base substitution occurred at a position 270 bp upstream of the ATG start codon in the BrTRUD1 promoter. This mutation significantly reduced the activity of the promoter and led to lower expression of BrTRUD1 in wdm21. Subcellular localization analysis displayed that BrTRUD1 localized to the nucleus. Heterologous complementation in Arabidopsis demonstrated that trud1 could restore to Col-0 via overexpressing BrTRUD1 in trud1. The research results demonstrated that BrTRUD1 mutant could lead to glossy leaf, which provided Chinese cabbage an excellent commercial trait.

Indexed as

BrassicaBrassica rapaIntramolecular TransferasesMutationPlant LeavesArabidopsisGene Expression Regulation, PlantPhenotypePlants, Genetically ModifiedPromoter Regions, GeneticIntramolecular Transferasespseudouridine synthases

Identifiers

PMID42426472

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.