Evidence map›Paper›PMID 42426453›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2026

Nanomechanical Phenotyping of Circulating Tumor Cells with Atomic Force Microscopy.

Pawel A Osmulski, Yusheng Qian, Maria Gaczynska

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Pawel A OsmulskiDepartment of Molecular Medicine, University of Texas Health Science Center at San Antonio, San Antonio, TX, USA. osmulski@uthscsa.edu.
Yusheng QianDepartment of Molecular Medicine, University of Texas Health Science Center at San Antonio, San Antonio, TX, USA.
Maria GaczynskaDepartment of Molecular Medicine, University of Texas Health Science Center at San Antonio, San Antonio, TX, USA.

Funding

Systems Analysis of Epigenomic Architecture in Cancer ProgressionU54CA217297 · NCI · UNIVERSITY OF TEXAS HLTH SCIENCE CENTER · PI WANG, QIANBEN · 2017 to 2021
$9.3M
The role of tumor-macrophage hybrid cells in prostate cancer metastasisU01CA283749 · NCI · UNIVERSITY OF TEXAS HLTH SCIENCE CENTER · PI Tim H.-M. Huang · 2024 to 2026
$1.9M
DOD PC170821NCI NIH HHS U01 CA283749NCI NIH HHS U54 CA217297
6 · The paper itself

Abstract

Tumor cells shed into the bloodstream are exposed to significant mechanical forces that are distinct from the native environment of epithelial-like cells. The surviving circulating tumor cells (CTCs) respond to circulation conditions with a range of mechanical adaptations resulting in their "mechanical fitness." Multiparameter nanomechanical phenotyping with atomic force microscopy (AFM)-based force spectrometry provides a comprehensive way to characterize these adaptations in a single-cell manner, in live CTCs isolated from the blood of cancer patients. Here, we describe the PeakForce Quantitative Nanomechanical (PF-QNM) phenotyping method for the determination of mechanical properties of CTCs in a fast and non-destructive way, permissive for additional downstream single-cell analyses.

Indexed as

Microscopy, Atomic ForceNanotechnologyNeoplastic Cells, CirculatingSingle-Cell AnalysisCell Line, TumorHumansPhenotypeAdhesionAtomic force microscopyCirculating tumor cellsDeformabilityElasticityForce spectrometryMechanical propertiesStiffness

Identifiers

PMID42426453

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.