ArticleMethods in molecular biology (Clifton, N.J.)2026
Androgen Receptor Splice Variant-7 Expression Analysis in Circulating Tumor Cells: Methodology and Application of AdnaTest in Prostate Cancer Research.
Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
This chapter outlines a detailed methodology for the analysis of androgen receptor splice variant-7 (AR-V7) expression in circulating tumor cells (CTCs) using the AdnaTestProstate CancerSelect and ProstateCancerDetect system. The method incorporates immunomagnetic enrichment, mRNA isolation, and reverse transcription PCR to detect AR-V7, a key marker associated with treatment resistance in castration-resistant prostate cancer (CRPC). The protocol includes step-by-step instructions for sample preparation, magnetic bead-based enrichment, and gene expression analysis, providing a robust platform to investigate the molecular characteristics of CTCs and their roles in therapy resistance.
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