Highly Efficient Capture of Sarcoma Cells in Microfluidic Devices.

Yang Zhang, Joanne P Lagmay, Z Hugh Fan

Abstract read

In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Yang ZhangDepartment of Mechanical and Aerospace Engineering, University of Florida, Gainesville, FL, 32611, USA.

Joanne P LagmayDepartment of Pediatrics, Division of Pediatric Hematology/Oncology, University of Florida, Gainesville, FL, 32610, USA.

Z Hugh FanPruitt Family Department of Mechanical and Aerospace Engineering, University of Florida, Gainesville, FL, 32611, USA. hfan@ufl.edu.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Circulating tumor cells (CTCs) can serve as a biomarker for cancer diagnosis, treatment management, and prognosis. However, their clinical utility is limited by the rarity and heterogeneity of CTCs in peripheral blood. Microfluidic technology has emerged as a powerful platform for the isolation, detection, and analysis of CTCs, offering high sensitivity and specificity. We have applied lateral filter array microfluidic (LFAM) devices for the enumeration of CTCs in blood samples of sarcoma patients. In this chapter, we detail the fabrication methods of the LFAM devices, with explanations of critical steps and practical considerations. Additionally, we discuss the techniques we employed for the capture and detection of sarcoma CTCs using these devices, highlighting their potential applications in clinical settings.

Indexed as

Cell SeparationLab-On-A-Chip DevicesMicrofluidic Analytical TechniquesNeoplastic Cells, CirculatingSarcomaBiomarkers, TumorCell Line, TumorHumansBiomarkers, TumorCirculating tumor cellsLateral filter arrayMicrofabricationMicrofluidicsSarcoma

Identifiers

PMID42426446

DOI10.1038/s41598-020-71041-7

What OpenQuestion holds

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.