Evidence map›Paper›PMID 42425083›Full record

ArticleImmunity2026

CRISPR-mediated precise large fragment insertion in zygotes enables rapid generation of humanized immunoglobulin heavy-chain mice.

Usha Nair, Madhav Akauliya, John E Warner, Shivang Parikh, Larance Ronsard, Maria Ssozi, Larissa Rays Wahba, Abigail G Esposito, Brendon Kelley, Caitlin McCarthy and 4 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Immunity, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Usha NairBatista Laboratory, The Ragon Institute of Mass General Brigham, Massachusetts Institute of Technology, and Harvard, Cambridge, MA 02139, USA. Electronic address: unair@mgh.harvard.edu.
Madhav AkauliyaBatista Laboratory, The Ragon Institute of Mass General Brigham, Massachusetts Institute of Technology, and Harvard, Cambridge, MA 02139, USA.
John E WarnerBatista Laboratory, The Ragon Institute of Mass General Brigham, Massachusetts Institute of Technology, and Harvard, Cambridge, MA 02139, USA.
Shivang ParikhBatista Laboratory, The Ragon Institute of Mass General Brigham, Massachusetts Institute of Technology, and Harvard, Cambridge, MA 02139, USA.
Larance RonsardLingwood Laboratory, The Ragon Institute of Mass General Brigham, Massachusetts Institute of Technology, and Harvard, Cambridge, MA 02139, USA.
Maria SsoziBatista Laboratory, The Ragon Institute of Mass General Brigham, Massachusetts Institute of Technology, and Harvard, Cambridge, MA 02139, USA.
Larissa Rays WahbaBatista Laboratory, The Ragon Institute of Mass General Brigham, Massachusetts Institute of Technology, and Harvard, Cambridge, MA 02139, USA.
Abigail G EspositoBatista Laboratory, The Ragon Institute of Mass General Brigham, Massachusetts Institute of Technology, and Harvard, Cambridge, MA 02139, USA.
Brendon KelleyBatista Laboratory, The Ragon Institute of Mass General Brigham, Massachusetts Institute of Technology, and Harvard, Cambridge, MA 02139, USA.
Caitlin McCarthyLingwood Laboratory, The Ragon Institute of Mass General Brigham, Massachusetts Institute of Technology, and Harvard, Cambridge, MA 02139, USA.
Stephanie R WeldonBatista Laboratory, The Ragon Institute of Mass General Brigham, Massachusetts Institute of Technology, and Harvard, Cambridge, MA 02139, USA.
Lin WuGenome Modification Facility, Department of Stem and Regenerative Biology, Harvard University, Cambridge, MA 02138, USA.
Daniel LingwoodLingwood Laboratory, The Ragon Institute of Mass General Brigham, Massachusetts Institute of Technology, and Harvard, Cambridge, MA 02139, USA.
Facundo D BatistaBatista Laboratory, The Ragon Institute of Mass General Brigham, Massachusetts Institute of Technology, and Harvard, Cambridge, MA 02139, USA; Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Department of Immunology, Harvard Medical School, Boston, MA 02115, USA. Electronic address: fbatista1@mgh.harvard.edu.

Funding

Accelerating Malaria Vaccines with a Custom Preclinical Humanized Mouse Model PlatformR01AI168114 · NIAID · MASSACHUSETTS GENERAL HOSPITAL · PI Facundo Damian Batista · 2022 to 2026
$3.5M
Triggering germline-encoded broadly neutralizing antibody responses against influenza virusR01AI153098 · NIAID · MASSACHUSETTS GENERAL HOSPITAL · PI BATISTA, FACUNDO DAMIAN, LINGWOOD, DANIEL · 2020 to 2023
$2.1M
Systemic coordination of pro-inflammatory immune reactions through dendritic cell-restricted sIL6R biogenesisR01AI155447 · NIAID · MASSACHUSETTS GENERAL HOSPITAL · PI LINGWOOD, DANIEL · 2021 to 2025
$2.0M
Elicitation of pan-influenza A antibodies via simple B cell development pathwaysR01AI195539 · NIAID · MASSACHUSETTS GENERAL HOSPITAL · PI Facundo Damian Batista, Daniel Lingwood · 2026 to 2026
$661k
Gates Foundation INV-046626Gates Foundation INV-085294NIAID NIH HHS R01 AI153098NIAID NIH HHS R01 AI155447NIAID NIH HHS R01 AI168114NIAID NIH HHS R01 AI195539
6 · The paper itself

Abstract

Current CRISPR-Cas9 methods are restricted to small genomic edits. We developed a CRISPR-guided approach that enables direct insertion of large genomic sequences into mouse zygotes. We deleted the murine 2.4-Mb immunoglobulin heavy-chain (IgH) variable (V

Indexed as

CRISPR-Cas SystemsImmunoglobulin Heavy ChainsZygoteAnimalsB-LymphocytesChromosomes, Artificial, BacterialHumansImmunoglobulin Variable RegionMiceMice, TransgenicSomatic Hypermutation, ImmunoglobulinV(D)J RecombinationImmunoglobulin Heavy ChainsImmunoglobulin Variable RegionBACbacterial artificial chromosomeCRISPR-Cas9genome editinghomology-directed repairhumanized antibodiesimmunoglobulinlarge DNA fragment knock-inmouse modelsSHMsomatic hypermutationzygote injection

Identifiers

PMID42425083
PMCPMC13361002

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.