ArticleMethods in molecular biology (Clifton, N.J.)2026
Integrating Maker Technology with Research: DIY Continuous Flow Colorimetric Detector with Integrated Data Acquisition and Processing.
Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Continuous flow analysis (CFA) offers a robust platform for real-time chemical monitoring and analysis, but the high cost and complexity of commercial systems limit their usage. This chapter describes a practical, low-cost, and reproducible approach for developing a continuous flow colorimetric detection system using open-source electronics, 3D-printed components, and custom data processing software. The setup incorporates a peristaltic pump, an Arduino-controlled solenoid valve, and an AS7341 visible light sensor aligned with a flow-through cuvette. A key innovation of this system is the integration of a Python-based algorithm to process real-time absorbance data and automatically identify and exclude interference-induced signal artifacts. This method enables low-cost detection of colorimetric changes in flowing samples and is particularly suited for biological and chemical analyses involving colorimetric detection, as well as educational settings. The system's modular design allows for easy customization, and its reproducibility makes it useful for dissemination across teaching laboratories or low-resource research environments.
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