Evidence map›Paper›PMID 42423794›Full record

ArticleArchives of microbiology2026

Enhancing 3-O-methylfunicone production by endophytic Talaromyces pinophilus J6 through culture optimization and its anti-Helicobacter pylori activity.

Marcus V A Marques, Dalila N Loose, Crislaine S Lima, Stéfane M Q Santos, Cecília L S Pereira, Gabriel S Ramos, Lorena C Queiroz, Rita C R Gonçalves, Eliane O Silva

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Archives of microbiology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Marcus V A Marques *Department of Organic Chemistry, Institute of Chemistry, Federal University of Bahia (UFBA), Barão de Jeremoabo 147, Salvador, Bahia, 40170-115, Brazil.
Dalila N Loose *Department of Pharmaceutical Sciences, Graduate Program in Pharmaceutical Sciences, Center of Health Sciences, Federal University of Espirito Santo, Vitoria, Espirito Santo, 29047-105, Brazil.
Crislaine S LimaDepartment of Organic Chemistry, Institute of Chemistry, Federal University of Bahia (UFBA), Barão de Jeremoabo 147, Salvador, Bahia, 40170-115, Brazil.
Stéfane M Q SantosDepartment of Organic Chemistry, Institute of Chemistry, Federal University of Bahia (UFBA), Barão de Jeremoabo 147, Salvador, Bahia, 40170-115, Brazil.
Cecília L S PereiraDepartment of Organic Chemistry, Institute of Chemistry, Federal University of Bahia (UFBA), Barão de Jeremoabo 147, Salvador, Bahia, 40170-115, Brazil.
Gabriel S RamosDepartment of Pharmaceutical Sciences, Graduate Program in Pharmaceutical Sciences, Center of Health Sciences, Federal University of Espirito Santo, Vitoria, Espirito Santo, 29047-105, Brazil.
Lorena C QueirozDepartment of Pharmaceutical Sciences, Graduate Program in Pharmaceutical Sciences, Center of Health Sciences, Federal University of Espirito Santo, Vitoria, Espirito Santo, 29047-105, Brazil.
Rita C R GonçalvesDepartment of Pharmaceutical Sciences, Graduate Program in Pharmaceutical Sciences, Center of Health Sciences, Federal University of Espirito Santo, Vitoria, Espirito Santo, 29047-105, Brazil. rita.goncalves@ufes.br.
Eliane O SilvaDepartment of Organic Chemistry, Institute of Chemistry, Federal University of Bahia (UFBA), Barão de Jeremoabo 147, Salvador, Bahia, 40170-115, Brazil. elianeos@ufba.br.ORCID https://orcid.org/0000-0001-9121-0481

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Endophytic fungi can produce metabolites with potential pharmaceutical applications through biosynthetic pathways strongly influenced by culture conditions. In this study, growth parameters (media composition and cultivation time) were systematically optimized to maximize 3-O-methylfunicone (3OMF) production by the endophytic fungus Talaromyces pinophilus J6. Chemical profiling by high-resolution mass spectrometry (HRMS) demonstrated that supplementation of potato dextrose agar (PDA) with 45% (w/w) ammonium sulfate and cultivation for 7 days significantly increased the accumulation of 3OMF compared to standard growth conditions. The optimized culture condition enabled the isolation of the target compound in sufficient yield for subsequent biological assays. The compound exhibited anti-Helicobacter pylori activity against one reference strain and three clinical isolates, with minimum inhibitory concentrations ranging from 0.165 to 0.660 mM and bactericidal concentrations up to 1.32 mM. In combination assays, 3OMF showed an additive effect with clarithromycin, indicating potential as an adjuvant compound in eradication therapies. Cytotoxicity assays of 3OMF demonstrated selective activity against gastric adenocarcinoma cells, with an IC

Indexed as

Anti-Bacterial AgentsEndophytesHelicobacter pyloriTalaromycesCell Line, TumorCulture MediaHumansMicrobial Sensitivity TestsMolecular Docking SimulationAnti-Bacterial AgentsCulture Media3-O-methylfuniconeCulture optimizationEndophytic fungiHelicobacter pyloriMolecular dockingTalaromyces pinophilus

Identifiers

PMID42423794
PMCPMC13350209

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.