Detection of ongoing asymptomatic

Background: Asymptomatic hosts can shed pathogens without showing clinical symptoms, making them invisible to routine screenings and potent drivers of pathogen dissemination and epidemic outbreaks. The lack of reliable, cost-effective tools for large-scale identification of asymptomatic infections hampers early intervention and control strategies. Methods: We improved the IgG serodynamics-based epitope discovery method by integrating clustering and high-level analysis, which helped us identify linear epitopes with immunogenicity from a large number of candidate epitopes. Epitopes were filtered using negative sera identified by virus neutralization tests (VNT) to eliminate highly antigenic probes. These remaining low antigenicity probes were used to construct a protein-peptide hybrid microarray (PPHM Results: The PPHM Conclusion: The study provides a novel, specific, and practical platform for detecting asymptomatic PDCoV infections based on serological TPI signatures. It offers early warning and disease prevention strategies in livestock and establishes a framework for future monitoring of potential interspecies transmission.