Evidence map›Paper›PMID 42421807›Full record

ArticlemicroPublication biology2026

KMT2C Knockdown impairs neuronal differentiation in human induced pluripotent stem cell-derived neural stem cells.

Masaya Ogawa, Shotaro Kawano, Sayaka Katayama, Naoto Ikeda, Rei Endo, Tomoki Mita, Yuuri Ikeuchi, Hotaka Fukushima, Daiki Miura, Takanobu Nakazawa

Abstract read
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In one paragraph

Article in microPublication biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Masaya OgawaTokyo University of Agriculture, Tokyo, 156-8502, Japan.
Shotaro KawanoTokyo University of Agriculture, Tokyo, 156-8502, Japan.
Sayaka KatayamaTokyo University of Agriculture, Tokyo, 156-8502, Japan.
Naoto IkedaTokyo University of Agriculture, Tokyo, 156-8502, Japan.
Rei EndoTokyo University of Agriculture, Tokyo, 156-8502, Japan.
Tomoki MitaTokyo University of Agriculture, Tokyo, 156-8502, Japan.
Yuuri IkeuchiTokyo University of Agriculture, Tokyo, 156-8502, Japan.
Hotaka FukushimaTokyo University of Agriculture, Tokyo, 156-8502, Japan.
Daiki MiuraTokyo University of Agriculture, Tokyo, 156-8502, Japan.
Takanobu NakazawaTokyo University of Agriculture, Tokyo, 156-8502, Japan.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

KMT2C mutations are strongly associated with autism spectrum disorder (ASD). Whereas multiplexed CRISPR screens show complete KMT2C knockout impairs neuronal differentiation, mixed-genotype pools cannot exclude non-autonomous paracrine effects. Furthermore, the functional effect of partial loss of function remains unclear. Here, we targeted KMT2C via shRNA knockdown in single-genotype cultures of human induced pluripotent stem cell-derived neural stem cells. Reduced KMT2C expression decreased the neuronal markers MAP2, HuC/D, and DCX. Our results demonstrate that partial, strictly cell-autonomous KMT2C insufficiency impairs neuronal stem cell differentiation into neurons, modeling the haploinsufficiency observed in patients to reveal a neurodevelopmental pathogenesis for ASD.

Identifiers

PMID42421807
PMCPMC13345183

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.