Evidence map›Paper›PMID 42421243›Full record

ArticleBiology of reproduction2026

Obesity alters the estrogen-induced luteinizing hormone surge in ovariectomized ewes†.

Trent Bronnenberg, Kelly S Kirkley, Christianne Magee, Dilyara A Murtazina, Whit C Stewart, Terry M Nett, Amy M Navratil, Jeremy D Cantlon, Brian D Cherrington, Colin M Clay

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biology of reproduction, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Trent BronnenbergDepartment of Zoology and Physiology, University of Wyoming, Laramie, WY 82071, USA.
Kelly S KirkleyDepartment of Biomedical Sciences, Colorado State University, Fort Collins, CO 80523, USA.
Christianne MageeDepartment of Biomedical Sciences, Colorado State University, Fort Collins, CO 80523, USA.
Dilyara A MurtazinaDepartment of Biomedical Sciences, Colorado State University, Fort Collins, CO 80523, USA.
Whit C StewartDepartment of Animal Science, University of Wyoming, Laramie, WY 82071, USA.
Terry M NettDepartment of Biomedical Sciences, Colorado State University, Fort Collins, CO 80523, USA.
Amy M NavratilDepartment of Zoology and Physiology, University of Wyoming, Laramie, WY 82071, USA.
Jeremy D CantlonDepartment of Biomedical Sciences, Colorado State University, Fort Collins, CO 80523, USA.ORCID 0000-0003-3408-2895
Brian D CherringtonDepartment of Zoology and Physiology, University of Wyoming, Laramie, WY 82071, USA.
Colin M ClayDepartment of Biomedical Sciences, Colorado State University, Fort Collins, CO 80523, USA.ORCID 0000-0003-4559-6656

Funding

Wyoming INBRE Phase 4- Equipment Supplement for x-ray diffractometer for Center for Advanced Scientific InstrumentationP20GM103432 · NIGMS · UNIVERSITY OF WYOMING · PI Nicolas A. Blouin · 2012 to 2026
$56.8M
Institutional Development AwardMultistate Research Project W-4112NIGMS NIH HHS P20 GM103432NIH HHS P20 GM103432USDA National Institute of Food and Agriculture Hatch program COLO0220CUSDA NIFA Animal Health and Disease NI22AHDRXXXXG011
6 · The paper itself

Abstract

Obesity is a leading health issue with a negative effect on fertility, but the complexity of the relationship between obesity and fertility requires further study. Santoro and colleagues have characterized the metabolic environment of obesity and the development of reprometabolic syndrome. Due to the reproductive similarities between ewes and women, we use ovariectomized ewes as a translational model of reprometabolic syndrome and tested whether obesity alters the estradiol-17β (E2)-induced luteinizing hormone (LH) surge. Each ovariectomized ewe serves as her own biological control during the three phases of the study: normal weight (phase 1), obese (phase 2), and a return to normal weight (phase 3). Ewes were weighed weekly, and 12th rib fat thickness was measured as a proxy for body condition score. During each of the three phases of the study, following an intramuscular injection of 25 μg of E2, serial blood samples were collected, and serum LH concentrations were measured by radioimmunoassay. The peak concentration and timing of the LH surge are significantly lower and delayed in obese ewes in phase 2 as compared to phases 1 and 3. Our results suggest that the mechanisms that underlie the pathological effects of obesity and reprometabolic syndrome on the E2-induced LH surge are similar in ewes and women. Therefore, ewes can serve as a model for parsing the relative roles of diminished hypothalamic and pituitary sensitivity to E2, resulting in a reduced and delayed LH surge associated with obesity and reprometabolic syndrome in women.

Indexed as

EstradiolEstrogensLuteinizing HormoneObesityOvariectomyAnimalsFemaleHypothalamic-Pituitary-Gonadal AxisSheepEstradiolEstrogensLuteinizing Hormoneestradiol positive feedbackgonadotropegonadotropin-releasing hormone receptorhyperinsulinemiametabolic regulation of reproductionneuroendocrine regulationpituitarypituitary sensitivityreprometabolic syndromesheep

Identifiers

PMID42421243
PMCPMC13578491

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.