Evidence map›Paper›PMID 42421159›Full record

ArticleEnvironmental microbiome2026

Beyond detection: unveiling microbial dynamics in oak seedlings using fungal isolation and amplicon sequencing.

Lucie Frejlichová, María Mercedes Maldonado-González, Petr Škarpa, Michal Tomšovský, Aleš Eichmeier

Abstract read
In one paragraph

Article in Environmental microbiome, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Lucie FrejlichováFaculty of Horticulture, Mendeleum - Institute of Genetics, Mendel University in Brno, Brno, Czech Republic.
María Mercedes Maldonado-GonzálezFaculty of Horticulture, Mendeleum - Institute of Genetics, Mendel University in Brno, Brno, Czech Republic.
Petr ŠkarpaDepartment of Agrochemistry, Soil Science, Microbiology and Plant Nutrition, Faculty of Agronomy, Mendel University in Brno, Brno, Czech Republic.
Michal TomšovskýDepartment of Forest Protection and Wildlife Management, Faculty of Forestry and Wood Technology, Mendel University in Brno, Brno, Czech Republic.
Aleš EichmeierFaculty of Horticulture, Mendeleum - Institute of Genetics, Mendel University in Brno, Brno, Czech Republic. ales.eichmeier@mendelu.cz.ORCID http://orcid.org/0000-0001-7358-3903

Funding

Internal Grant Schemes of Mendel University in Brno, Internal Grant Agency of the Faculty of Forestry and Wood Technology LDF_VP_2021028Ministerstvo Školství, Mládeže a Tělovýchovy CZ.02.1.01/0.0/0.0/16_017/0002334
6 · The paper itself

Abstract

backgroundForest nurseries are critical for producing resilient nursery stock for reforestation and planting of ornamental trees, yet the microbial communities associated with nursery grown plants remain poorly characterized. Quercus robur L. seedlings from seven Czech forest nurseries were analyzed to assess microbial diversity, co-occurrence patterns, and environmental drivers.

resultsMicrobial communities were characterized using fungal ITS2 and bacterial 16S rRNA gene amplicon sequencing, fungal isolation, and comprehensive soil chemistry. HTAS revealed broader taxonomic and functional profiles compared to isolation, which selectively enriched for fast growing pathogens. Both methods were found to be complementary, emphasizing the value of methodological integration. Fungal communities exhibited pronounced site specific beta diversity and responded to inorganic soil parameters, particularly calcium, phosphorus, and pH/CaCl

conclusionsMineral soil properties were associated with microbial community structure and fungal trophic composition. Despite standardized nursery conditions, edaphic variability exerted strong filtering effects on fungal and bacterial communities. These findings provide ecological insight into seedling microbe interactions and offer a basis for microbiome informed nursery management strategies.

Indexed as

Amplicon sequencingFungal isolationMicrobial associationsSoil chemistrySymptomatic oak seedlings

Identifiers

PMID42421159
PMCPMC13628951

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.