Evidence map›Paper›PMID 42420947›Full record

ArticleBMC biotechnology2026

Process optimization for protoplast preparation from Grifola frondosa and development of a high-yield mutant with enhanced mycelial growth and protein production.

Qiaoyu Wang, Ziteng Zhao, Qiuyu Lu, Ke'er Xiao, Lingyu Gao, Mukaddas Sai, Lili Guan, Linna Du

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC biotechnology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Qiaoyu Wang *Engineering Research Center of Bioreactor and Pharmaceutical Development, Ministry of Education, College of Life Science, Jilin Agricultural University, No. 2888, Xincheng Street, Changchun, 130118, China.
Ziteng Zhao *Engineering Research Center of Bioreactor and Pharmaceutical Development, Ministry of Education, College of Life Science, Jilin Agricultural University, No. 2888, Xincheng Street, Changchun, 130118, China.
Qiuyu LuEngineering Research Center of Bioreactor and Pharmaceutical Development, Ministry of Education, College of Life Science, Jilin Agricultural University, No. 2888, Xincheng Street, Changchun, 130118, China.
Ke'er XiaoEngineering Research Center of Bioreactor and Pharmaceutical Development, Ministry of Education, College of Life Science, Jilin Agricultural University, No. 2888, Xincheng Street, Changchun, 130118, China.
Lingyu GaoEngineering Research Center of Bioreactor and Pharmaceutical Development, Ministry of Education, College of Life Science, Jilin Agricultural University, No. 2888, Xincheng Street, Changchun, 130118, China.
Mukaddas SaiEngineering Research Center of Bioreactor and Pharmaceutical Development, Ministry of Education, College of Life Science, Jilin Agricultural University, No. 2888, Xincheng Street, Changchun, 130118, China.
Lili GuanEngineering Research Center of Bioreactor and Pharmaceutical Development, Ministry of Education, College of Life Science, Jilin Agricultural University, No. 2888, Xincheng Street, Changchun, 130118, China. llguan@jlau.edu.cn.
Linna DuEngineering Research Center of Bioreactor and Pharmaceutical Development, Ministry of Education, College of Life Science, Jilin Agricultural University, No. 2888, Xincheng Street, Changchun, 130118, China. dulinna0918@163.com.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Grifola frondosa is a highly valued medicinal mushroom with diverse bioactive properties; however, its low mycelial biomass remains a major bottleneck affecting the efficiency of liquid fermentation. To address this issue, protoplast-mediated mutagenesis was employed in this study to breed a high-performance G. frondosa mutant with superior fermentation traits. Following optimization using intelligent strategies, the highest yield of protoplasts was achieved (6.573 × 10

Indexed as

Fungal ProteinsGrifolaMyceliumProtoplastsBiomassFermentationMutagenesisMutationFungal ProteinsGrifola frondosaMutagenesisOptimizationProductionProtoplast

Identifiers

PMID42420947
PMCPMC13628877

What OpenQuestion holds

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Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.