Cell-Free Genetic Circuits: Extract and Template Preparation.

Thales R Spartalis, Kassidy B Porche, Xun Tang, Yong-Chan Kwon

Abstract read

In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Thales R SpartalisCain Department of Chemical Engineering, Louisiana State University, Baton Rouge, LA, USA.

Kassidy B PorcheDepartment of Biological and Agricultural Engineering, Louisiana State University, Baton Rouge, LA, USA.

Xun TangCain Department of Chemical Engineering, Louisiana State University, Baton Rouge, LA, USA. xuntang@lsu.edu.

Yong-Chan KwonCain Department of Chemical Engineering, Louisiana State University, Baton Rouge, LA, USA. yckwon@lsu.edu.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Cell-free systems have been highlighted as a transformative technology in synthetic biology, offering substantial technological advantages over traditional in vivo methods. By leveraging cellular metabolic machinery of the cells and decoupling cell growth from catalytic utilization and flexible template configuration, cell-free systems allow direct access to cell machinery and facilitate precise control over reaction conditions. Despite these advantages, standardizing DNA template preparation and cellular extract production remains a considerable challenge, often leading to variability in results. This chapter details methods for preparing cellular extracts and outlines strategies for optimizing DNA template preparation to enhance the reliability and efficiency of cell-free synthetic gene circuits.

Indexed as

DNAGene Regulatory NetworksSynthetic BiologyCell-Free SystemTemplates, GeneticDNACell-free systemsCellular extractDNA template preparationGenetic circuitsSynthetic biology

Identifiers

PMID42420729

DOI10.1021/sb400131a

What OpenQuestion holds

Textmetadata

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.