ArticleMethods in molecular biology (Clifton, N.J.)2026
Design of Conditional Guide RNAs for the Logical Regulation of Gene Expression.
Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The CRISPR interference (CRISPRi) is an RNA-guided regulator that silences gene expression by binding to its cognate DNA target, halting transcription in both prokaryotic and eukaryotic cells. Recent advances in RNA synthetic biology have endowed CRISPR guide RNAs (gRNAs) with conditional functionality: these so-called conditional guide RNAs (cgRNAs) fold into strong hairpins that block their activity until a specific trigger RNA is present. Upon introduction of the cognate trigger RNAs, the hairpin structure unfolds, allowing the activated cgRNA to direct transcriptional repression with large dynamic ranges, minimal crosstalk, expanded tunability, and logic-gated signal processing. Furthermore, cgRNAs can be integrated into endogenous gene circuits to achieve sophisticated and logical regulation of gene expression. This chapter describes the design of cgRNAs and provides detailed protocols for their in vivo characterization in E. coli.
Indexed as
Identifiers
42420725What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.