Evidence map›Paper›PMID 42420631›Full record

ArticleTropical animal health and production2026

Dietary sesame seed oil supplementation enhances productive performance, antioxidant status, serum lipids, intestinal morphology and microbial load in rhode island red laying hens.

Saba Ajam, Sar Zamin Khan, Rafi Ullah, Shakoor Ahmad, Naseer Ahmad, Rifat Ullah Khan

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Article in Tropical animal health and production, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

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No citing paper in PubMed yet.

4 · The record

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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Saba AjamDepartment of Poultry Science, Faculty of Animal Husbandry and Veterinary Sciences, The University of Agriculture, Peshawar, Pakistan.
Sar Zamin KhanDepartment of Poultry Science, Faculty of Animal Husbandry and Veterinary Sciences, The University of Agriculture, Peshawar, Pakistan.
Rafi UllahDepartment of Poultry Science, Faculty of Animal Husbandry and Veterinary Sciences, The University of Agriculture, Peshawar, Pakistan.
Shakoor AhmadCollege of Veterinary Sciences, Faculty of Animal Husbandry and Veterinary Sciences, The University of Agriculture, Peshawar, Pakistan.
Naseer AhmadDepartment of Poultry Science, Faculty of Animal Husbandry and Veterinary Sciences, The University of Agriculture, Peshawar, Pakistan.
Rifat Ullah KhanCollege of Veterinary Sciences, Faculty of Animal Husbandry and Veterinary Sciences, The University of Agriculture, Peshawar, Pakistan. rukhan@aup.edu.pk.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

This study evaluated the effects of dietary sesame oil (SO) supplementation on production performance, egg quality, serum biochemical indices, antioxidant status, intestinal microflora, and intestinal morphology of laying hens. A total of 240 healthy, 30-week-old Rhode Island Red (RIR W-30) hens were randomly allotted to four dietary treatments in a completely randomized design, with five replicates per treatment (12 birds per replicate), over a five-week experimental period. The treatments consisted of a control diet without SO and diets supplemented with 0.5, 1.0, or 1.5 mL/kg SO. Dietary SO supplementation significantly improved production performance, as evidenced by increased egg production (daily and weekly), higher hen-day egg production, and reduced feed conversion ratio, with the greatest improvements observed at the highest inclusion level. Feed intake increased marginally but remained within normal physiological limits. Egg quality traits, including egg weight, shell characteristics, albumen height, and Haugh unit, were not affected by SO supplementation. Serum lipid profile was favorably modulated, with significant reductions in low-density lipoprotein and total cholesterol and a marked increase in high-density lipoprotein cholesterol, while triglyceride levels remained unchanged. Antioxidant status was significantly enhanced, as indicated by increased activities of superoxide dismutase and glutathione peroxidase and elevated total antioxidant status. Furthermore, SO supplementation reduced intestinal counts of Salmonella spp. and Escherichia coli and improved intestinal morphology by increasing villus height and villus-to-crypt ratio, particularly in the duodenum. In conclusion, dietary sesame oil, especially at 1.5 mL/kg, can be used as a functional feed additive to enhance productivity, metabolic health, antioxidant capacity, and gut health in laying hens without compromising egg quality.

Indexed as

AntioxidantsChickensGastrointestinal MicrobiomeIntestinesLipidsSesame OilAnimal FeedAnimal Nutritional Physiological PhenomenaAnimalsDietDietary SupplementsEggsFemaleRandom AllocationAntioxidantsLipidsSesame OilAntioxidant statusIntestinal morphologyLaying hensSerum lipid profileSesame oil supplementation

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.