Evidence map›Paper›PMID 42420473›Full record

ArticleInternational journal of legal medicine2026

Evaluation of the RTyper Y27 chip amplification system with Quick TargSeq 1.0 for Y-STR genotyping in forensic science.

Junping Han, Yiren Yao, Qun Wan, Xuanzhu Chen, Junyi Shen, Boxu Chen, Yongjie Li, Bin Zhuang, Lijian Zhao, Lan Hu and 1 more

Abstract readEvaluation Study
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In one paragraph

Article in International journal of legal medicine, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Junping HanCriminal Investigation Detachment, Chaoyang District Branch, Beijing Public Security Bureau, Beijing, 100025, China.
Yiren YaoKey Laboratory of Forensic Genetics, Beijing Engineering Research Center for Crime Scene Evidence Examination, National Engineering Laboratory for Forensic Science, Institute of Forensic Science, Muxidinanli 17, Xicheng District, 100038, Beijing, People's Republic Of China.
Qun WanDepartment of Radiology, 960th Hospital of the PLA Joint Logistics Support Force, Jinan, 250031, Shandong, China.
Xuanzhu ChenJiangsu Key Laboratory of Phylogenomics and Comparative Genomics, School of Life Science, Jiangsu International Joint Research Center of Genomics, Jiangsu Normal University, Xuzhou, 221116, Jiangsu, People's Republic of China.
Junyi ShenInstitute of Forensic Sciences Public Security Department of Shanxi, Taiyuan, 030001, Shanxi, China.
Boxu ChenPeople's Public Security University of China, Beijing, 100038, China.
Yongjie LiPeople's Public Security University of China, Beijing, 100038, China.
Bin ZhuangBeijing CapitalBio Technology Co., Ltd, Beijing, 101111, China.
Lijian ZhaoBeijing CapitalBio Technology Co., Ltd, Beijing, 101111, China.
Lan HuKey Laboratory of Forensic Genetics, Beijing Engineering Research Center for Crime Scene Evidence Examination, National Engineering Laboratory for Forensic Science, Institute of Forensic Science, Muxidinanli 17, Xicheng District, 100038, Beijing, People's Republic Of China. hulan328@139.com.
Caixia LiKey Laboratory of Forensic Genetics, Beijing Engineering Research Center for Crime Scene Evidence Examination, National Engineering Laboratory for Forensic Science, Institute of Forensic Science, Muxidinanli 17, Xicheng District, 100038, Beijing, People's Republic Of China. licaixia@tsinghua.org.cn.

Funding

Special Program of Basic Research for Central Public Welfare Research Institutes 2024JB025the Open Subject of the Beijing Engineering Research Center of Crime Scene Evidence Examination 2021CSEEKFKT06
6 · The paper itself

Abstract

The male-specific Y chromosome is widely used in forensic DNA analysis. High-mutation Y-chromosomal short tandem repeats (Y-STRs) increase the ability to differentiate related males, enhancing discrimination capacity. Current Y-STR typing requires trained forensic personnel, specialized instruments, and a controlled lab environment, taking 6-8 h. The Quick TargSeq 1.0 system integrates DNA extraction, PCR amplification, and electrophoresis via microfluidic biochip technology. Our previous study showed that Quick TargSeq can automatically produce auto-STR and DIP-38 profiles from buccal swabs, blood stains, or other biological samples in about 2 h. Here, we developed a Y-STR kit for Quick TargSeq. The RTyper Y27 microfluidic amplification system is a five-dye multiplex setup containing 27 core Y-STR loci (DYS19, DYS385a/b, DYS389I, DYS389II, DYS390, DYS391, DYS392, DYS393, DYS437, DYS438, DYS439, DYS448, DYS456, DYS458, DYS481, DYS533, DYS576, DYS635, Y-GATA-H4, DYF387S1a/b, DYS449, DYS460, DYS518, DYS627, and DYS570). To confirm the system's efficiency and accuracy, we conducted sensitivity, concordance, reproducibility, inhibition, mixture, and versatility studies following SWGDAM developmental validation guidelines. Results demonstrate that the RTyper Y27 system is efficient, accurate, and reliable for forensic applications.

Indexed as

Chromosomes, Human, YDNA FingerprintingMicrosatellite RepeatsGenotypeHumansMalePolymerase Chain ReactionForensic geneticsMicrofluidic biochipRapid DNA (RDNA)Short tandem repeatY chromosome

Identifiers

PMID42420473

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.