ArticleAMB Express2026
Solid-state fermentation of tropical agro-industrial wastes using Pleurotus ostreatus for fungal protein production.
Article in AMB Express, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Solid-state fermentation (SSF) of lignocellulosic agro-industrial wastes using white-rot fungi represents a scalable, low-energy strategy for converting underutilised tropical biomass into high-quality fungal protein. This study optimised six substrate formulations combining cocoa pod husk (CPH), hardwood sawdust, and composted vegetable waste for Pleurotus ostreatus NIHORT EM-1 (ITS rDNA authenticated; GenBank MN165852; 99.8% identity to CBS 244.31) under Nigerian tropical conditions. The strain is deposited at the National Horticultural Research Institute (NIHORT), Ibadan, Nigeria, and is publicly available for research purposes upon written request to the Director of Research, NIHORT, P.M.B. 5432, Idi-Ishin, Ibadan, Nigeria (email: info@nihort.gov.ng; Tel: +234-803-000-0001; online germplasm catalogue: www.nihort.gov.ng/germplasm ). Substrate carbon-to-nitrogen (C:N) ratio, mycelial colonisation kinetics, fresh-weight yield, proximate nutritional quality (protein digestibility-corrected amino acid score, PDCAAS), and contamination incidence were evaluated across a completely randomised design (n = 5 replicates per treatment; 30 bags total). The optimal formulation [T5: 58% sawdust, 25% CPH, 10% composted vegetable waste, 5% rice bran; C:N 31.2:1] achieved biological efficiency (BE) of 116.9%, protein yield of 359 g kg⁻
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