Evidence map›Paper›PMID 42418051›Full record

ArticleMolecular biology reports2026

Naringenin attenuates amiodarone-induced pulmonary fibrosis in rats via modulation of the SIRT1/NF-κB signalling pathway.

Heba M Arakeep, Wafaa Abdelaziz Emam, Amena Rezk Mohammed, Marwa H Sedira, Marwa Mahmoud Awad, Alshimaa Aboalsoud, Rehab Ahmed Ahmed El-Shaer, Azza Mostafa Mohammed Aboshanady, Norhan Ahmed AbuoHashish

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Article in Molecular biology reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Not yet cited in PubMed.

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1 · What the graph read from it

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4 · The record

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5 · Who and what money

Authors and funding

9 authors.

Heba M ArakeepAnatomy & Embryology Department, Faculty of Medicine, Tanta University, Tanta, Egypt.
Wafaa Abdelaziz EmamMedical Biochemistry Department, Faculty of Medicine (Girls), Al-Azhar University, Cairo, Egypt. wafaaabdelaziz.medg@azhar.edu.eg.
Amena Rezk MohammedMedical Biochemistry Department, Faculty of Medicine (Girls), Al-Azhar University, Cairo, Egypt. amenarezk.medg@azhar.edu.eg.
Marwa H SediraMedical Biochemistry Department, Faculty of Medicine (Girls), Al-Azhar University, Cairo, Egypt.
Marwa Mahmoud AwadPhysiology Department, Faculty of Medicine, Tanta University, Tanta, 31527, Egypt.
Alshimaa AboalsoudPharmacology Department, Faculty of Medicine, TantaUniversity, Tanta, Egypt.
Rehab Ahmed Ahmed El-ShaerPhysiology Department, Faculty of Medicine, Tanta University, Tanta, 31527, Egypt.
Azza Mostafa Mohammed AboshanadyAnatomy & Embryology Department, Faculty of Medicine, Tanta University, Tanta, Egypt.
Norhan Ahmed AbuoHashishPharmacology Department, Faculty of Medicine, TantaUniversity, Tanta, Egypt.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundAmiodarone (AMD)-induced pulmonary fibrosis (PF) is a serious adverse effect driven by oxidative stress and inflammation. Naringenin [NAR], a citrus flavonoid, possesses potent antioxidant and anti-inflammatory properties. This study investigated the protective effects of NAR against AMD-induced PF in rats, focusing on the sirtuin 1; nuclear factor Kappa -B (SIRT1/NF-κB) pathway.

methodsThirty-two male Wistar rats were divided into four experimental groups (n = 8): control (CON), (NAR), (AMD), and AMD + NAR. In the AMD groups, PF was induced using AMD. NAR treatment was administered daily via oral gavage for five weeks.

resultsAfter 5 weeks, the AMD group exhibited significant alveolar damage and collagen deposition. Biochemically, AMD induced severe oxidative stress, characterized by increased malondialdehyde and nitric oxide (NO) levels. It also depleted antioxidant defences, including reduced glutathione (GSH) and superoxide dismutase (SOD). A robust inflammatory response was observed, with elevated levels of tumor necrosis factor -alpha (TNF-α), (NF-κB), and interleukin-6 (IL-6). The profibrotic cascade was activated through increased transforming growth factor beta 1(TGF-β1) and matrix metalloproteinase (MMP-2), alongside the upregulation of connective tissue growth factor (CTGF) and platelet derived growth factor (PDGF) mRNA. Apoptotic signalling was heightened by decreased B-cell lymphoma2 (Bcl-2) levels. Crucially, AMD downregulated SIRT1 and mitogen activated protein kinase (MAPK1/ERK2) mRNA expression. Conversely, NAR co-treatment significantly attenuated histopathological changes and reduced fibrosis scores. NAR effectively mitigated oxidative stress and restored antioxidant defenses. It also suppressed inflammatory and modulates anti-apoptotic signalling pathways. Notably, NAR decreased TGF-β1, MMP-2, CTGF, and PDGF, while restoring SIRT1 and MAPK1/ERK2 expression.

conclusionsNAR provides significant protection against AMD-induced PF by reducing oxidative stress, inflammation, and modulating anti-apoptotic signalling pathways. These effects are likely mediated through the modulation of the SIRT1/NF-κB signalling pathway. This suggests NAR as a potential therapeutic candidate for drug-induced lung injury.

Indexed as

AmiodaroneFlavanonesNF-kappa BPulmonary FibrosisSirtuin 1AnimalsAntioxidantsMaleOxidative StressRatsRats, WistarSignal TransductionAmiodaroneAntioxidantsFlavanonesnaringeninNF-kappa BSirt1 protein, ratSirtuin 1AmiodaroneNaringeninOxidative stressPulmonary fibrosisRat modelSIRT1/NF-κB pathway

Identifiers

PMID42418051

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.