ArticleJPhys photonics2026
FLIMExplorer: interactive GUI for object-based visualization and analysis of fluorescence lifetime images.
Article in JPhys photonics, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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6 authors.
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Abstract
Fluorescence lifetime imaging microscopy (FLIM) enables non-invasive measurement of cellular metabolism with single-cell and subcellular resolution, providing advantages over traditional population-level metabolic assays. However, current single-cell FLIM analysis workflows typically rely on multiple software tools for lifetime extraction, segmentation, and downstream analysis, often resulting in convoluted workflows and a disconnect between quantitative measurements and image context, limiting transparent quality control. Here, we present FLIMExplorer, an interactive, Python-based tool for downstream single-cell FLIM analysis and visualization. FLIMExplorer links quantitative FLIM endpoints and their underlying image objects, enabling users to explore key FLIM features (NAD(P)H and FAD:
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