Evidence map›Paper›PMID 42416279›Full record

ArticleFrontiers in cellular and infection microbiology2026

Rapid and low-cost detection of six major pediatric enteric pathogens using a closed-tube PCR-invasive reaction assay.

Nan Yang, Jin Zhou, Han Zhang, Yihsuan Huang, Guiping Kong, Yucan Zheng, Zhuo Zhang, Ying Shen, Xiu Han, Zhifeng Liu and 1 more

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In one paragraph

Article in Frontiers in cellular and infection microbiology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Nan Yang *Department of Gastroenterology, Children's Hospital of Nanjing Medical University, Nanjing, China.
Jin Zhou *Department of Clinical Laboratory, Children's Hospital of Nanjing Medical University, Nanjing, China.
Han ZhangDepartment of Gastroenterology, Children's Hospital of Nanjing Medical University, Nanjing, China.
Yihsuan HuangDepartment of Gastroenterology, Children's Hospital of Nanjing Medical University, Nanjing, China.
Guiping KongDepartment of Gastroenterology, Children's Hospital of Nanjing Medical University, Nanjing, China.
Yucan ZhengDepartment of Gastroenterology, Children's Hospital of Nanjing Medical University, Nanjing, China.
Zhuo ZhangDepartment of Gastroenterology, Children's Hospital of Nanjing Medical University, Nanjing, China.
Ying ShenDepartment of Gastroenterology, Children's Hospital of Nanjing Medical University, Nanjing, China.
Xiu HanSchool of Basic Medical Sciences, Faculty of Medicine, Yangzhou University, Yangzhou, China.
Zhifeng LiuDepartment of Gastroenterology, Children's Hospital of Nanjing Medical University, Nanjing, China.
Yan LuDepartment of Gastroenterology, Children's Hospital of Nanjing Medical University, Nanjing, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Diarrhea caused by bacterial and viral pathogens remains a major cause of childhood morbidity and mortality, leading to dehydration, malnutrition, and substantial healthcare burdens; rapid and accurate diagnosis is therefore essential for timely treatment. In clinical practice, detection still commonly relies on traditional methods such as stool culture and enzyme-linked immunosorbent assays. These approaches are not only time-consuming and lack sensitivity but also typically require separate, pathogen-specific tests, complicating comprehensive diagnosis. While quantitative PCR (qPCR) offers speed and sensitivity, dye-based methods lack specificity, and fluorescent probe-based assays are costly. To address these limitations, we developed an integrated assay that combines PCR with a sequence-specific invasive reaction (Invader) in a single closed tube. This method detects six major pediatric enteric pathogens:

Indexed as

DiarrheaMolecular Diagnostic TechniquesPolymerase Chain ReactionCampylobacter jejuniFecesHumansRapid Diagnostic TestsRotavirusSensitivity and SpecificityShigellaVibrio choleraeclosed-tube assayco-infection detectionenteric pathogensinvasive reactionrapid and low-cost detection

Identifiers

PMID42416279
PMCPMC13337639

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.