ArticleJournal of neuroendocrinology2026
Single-nuclei RNA sequencing reveals heterogeneity within developing GnRH3 neurons in zebrafish.
Article in Journal of neuroendocrinology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Gonadotropin-releasing hormone (GnRH) is a central regulator of vertebrate reproduction regulating pituitary gonadotropins. In zebrafish, GnRH3 serves as the hypophysiotropic isoform. Its neurons develop by migrating from the nasal placode to the hypothalamus, forming several distinct subpopulations along the migratory path. However, the molecular heterogeneity underlying this migration remains incompletely understood. To delineate the molecular and spatial heterogeneity, we employed single-nuclei RNA sequencing of nuclei enriched based on gnrh3 promoter-driven EGFP fluorescence from 7 dpf Tg(gnrh3:EGFP) zebrafish. This was followed by GnRH3 neurons clustering, differentially expressed genes identification, trajectory inference as a hypothesis-generating framework, and functional analyses. Marker genes were selected and used for validation and to determine the distribution of each subcluster by in situ hybridization (ISH) and immunohistochemistry (IHC) in larval and adult zebrafish. Three subpopulations were identified featuring highly expressed marker genes: Gnrh3
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