Evidence map›Paper›PMID 42415166›Full record

ArticleJournal of animal science and biotechnology2026

Systems metabolic engineering of Corynebacterium glutamicum for efficient production of L-isoleucine.

Junkun Cao, Ming Huang, Qi Sheng, Landong Zhang, Gang Meng, Chunguang Zhao, Aiying Wei, Jia Liu, Liming Liu

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of animal science and biotechnology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Junkun Cao *School of Food Science and Engineering, Anhui Science and Technology University, Chuzhou, Anhui, 239000, China.
Ming Huang *School of Biotechnology and Key Laboratory of Industrial Biotechnology of Ministry of Education, Jiangnan University, Wuxi, Jiangsu, 214122, China.
Qi ShengSchool of Biotechnology and Key Laboratory of Industrial Biotechnology of Ministry of Education, Jiangnan University, Wuxi, Jiangsu, 214122, China.
Landong ZhangSchool of Life Sciences and Health Engineering, Jiangnan University, Wuxi, Jiangsu, 214122, China.
Gang MengNingxia Eppen Biotech Co., Ltd., Yinchuan, Ningxia, 750000, China.
Chunguang ZhaoNingxia Eppen Biotech Co., Ltd., Yinchuan, Ningxia, 750000, China.
Aiying WeiNingxia Eppen Biotech Co., Ltd., Yinchuan, Ningxia, 750000, China.
Jia LiuSchool of Biotechnology and Key Laboratory of Industrial Biotechnology of Ministry of Education, Jiangnan University, Wuxi, Jiangsu, 214122, China.
Liming LiuSchool of Food Science and Engineering, Anhui Science and Technology University, Chuzhou, Anhui, 239000, China. mingll@jiangnan.edu.cn.

Funding

Jiangsu Agricultural Science and Technology Innovation Fund CX(22)1012Regional Joint Fund of the National Natural Science Foundation of China U25A20618
6 · The paper itself

Abstract

backgroundL-Isoleucine is an essential branched-chain amino acid for livestock and poultry, supporting protein accretion and regulating energy metabolism, immune function, and stress resilience.

resultsA genetically stable L-isoleucine producer, Corynebacterium glutamicum cgl-Ile0, was obtained via biosensor-assisted ARTP mutagenesis, and produced 11.52 g/L L-isoleucine in a 5-L fermenter, with a yield of 0.11 g/g and a productivity of 0.24 g/L/h. Whole-genome resequencing revealed four mutations associated with the phenotype, including aspB

conclusionsWe developed an industrial L-isoleucine-producing C. glutamicum strain by integrating biosensor-guided ARTP mutagenesis, structure-guided protein engineering, and modular pathway rewiring, providing a practical and transferable framework for constructing GRAS amino-acid producers for animal nutrition.

Indexed as

Atmospheric and room-temperature plasma (ARTP) mutagenesisCorynebacterium glutamicumFed-batch fermentationL-IsoleucineModular metabolic engineering

Identifiers

PMID42415166
PMCPMC13343988

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LicenceCC BY
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.