Evidence map›Paper›PMID 42415144›Full record

ArticleStem cell research & therapy2026

Isolation of human satellite cells using CD29/CDH15 and a nanoparticle-based live Pax7 mRNA detection system, and their expansion with extracellular matrix and its derivatives.

Shudong Zhao, Shubhankar Sood, Ruixiang Yu, Luis Arvizu Gutierrez, Takhmina Ayupova, Sara Taiyari, Jonathan Miles, Stephen Hamilton, Massimiliano Cerletti

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Stem cell research & therapy, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Shudong ZhaoDivision of Surgery and Interventional Science, Tissue Repair and Regeneration Centre, University College London, Royal Free Campus, London, NW3 2PF, UK.
Shubhankar SoodDivision of Surgery and Interventional Science, Tissue Repair and Regeneration Centre, University College London, Royal Free Campus, London, NW3 2PF, UK.
Ruixiang YuDivision of Surgery and Interventional Science, Tissue Repair and Regeneration Centre, University College London, Royal Free Campus, London, NW3 2PF, UK.
Luis Arvizu GutierrezDivision of Surgery and Interventional Science, Tissue Repair and Regeneration Centre, University College London, Royal Free Campus, London, NW3 2PF, UK.
Takhmina AyupovaDivision of Surgery and Interventional Science, Tissue Repair and Regeneration Centre, University College London, Royal Free Campus, London, NW3 2PF, UK.
Sara TaiyariDivision of Surgery and Interventional Science, Tissue Repair and Regeneration Centre, University College London, Royal Free Campus, London, NW3 2PF, UK.
Jonathan MilesOrthopaedic Surgery, The Royal National Orthopaedic Hospital, Brockley Hill, Stanmore, HA7 4AP, UK.
Stephen HamiltonDivision of Surgery and Interventional Science, Tissue Repair and Regeneration Centre, University College London, Royal Free Campus, London, NW3 2PF, UK.
Massimiliano CerlettiDivision of Surgery and Interventional Science, Tissue Repair and Regeneration Centre, University College London, Royal Free Campus, London, NW3 2PF, UK. m.cerletti@ucl.ac.uk.

Funding

Wellcome Trust
6 · The paper itself

Abstract

Human skeletal muscle stem cells, also called satellite cells, have great potential for treating neuromuscular diseases and combating muscle aging. However, progress toward clinical use has been limited by the scarcity of donor tissue and the technical challenges of isolating, preserving, and culturing these rare cells. In this study, we introduce a new combination of cell surface markers and a gold nanoparticle-based live Pax7 mRNA detection system, both of which enable efficient isolation of human satellite cells. Additionally, we show that extracellular matrix (ECM) components support the maintenance of human satellite cells and improve their function in vivo. These findings provide integrated methods to enhance the production of human muscle-forming cells for translational and clinical applications.

Indexed as

CadherinsCell SeparationExtracellular MatrixPAX7 Transcription FactorRNA, MessengerSatellite Cells, Skeletal MuscleAnimalsGoldHumansMetal NanoparticlesMuscle, SkeletalCadherinsGoldPAX7 protein, humanPAX7 Transcription FactorRNA, MessengerCell surface markersDecellularizationECMHuman satellite cellsNanoparticle-based live mRNA detection probesSkeletal muscle regeneration

Identifiers

PMID42415144
PMCPMC13637147

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.