ArticleJournal of translational medicine2026
hsa_circ_0063865 regulates cytoskeleton rearrangement and endoplasmic reticulum stress to promote esophageal tumorigenesis under co-exposure to N-nitrosamines and microcystin.
Article in Journal of translational medicine, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
backgroundHigh enrichment of N-nitrosamines (NAs) and microcystins (MCs) in drinking water are major risk factors in high-risk areas of esophageal squamous cell carcinoma (ESCC) in China, however, their combined effects and underlying carcinogenic mechanisms remain unknown.
methodsThe scRNA_seq sequencing and CTD database were used to identify the "chemical-epithelial cell" associated carcinogenic targets. An ESCC rat model was established to confirm the combined effect of NAs and MCs; RNA-Seq was performed to screen potential circRNAs in ESCC and matched adjacent normal tissues. Malignant transformation of esophageal epithelial cells (Het-1A-T) was induced by exposure to N-nitrosomethylbenzylamine (NMBzA) and Microcystin-LR (MC-LR). The biological function of hsa_circ_0063865 was investigated using gain- and loss-of-function experiments in vitro and in vivo. RNA pull-down, ChIRP, RIP, Co-IP and luciferase reporter assays were used to elucidate the underlying mechanisms of hsa_circ_0063865 in chemically related ESCC.
resultsThe rat ESCC model and median-effect principle showed a synergistic effect of NAs and MC-LR in chemical carcinogenesis. Further, integration of single-cell and network toxicology revealed 292 "chemical-epithelial cell" associated carcinogenic targets, which were primarily involved in microRNA in cancer and cytoskeleton rearrangements. At the molecular level, the chemical carcinogenesis-related hsa_circ_0063865 was identified and found to be up-regulated in ESCC tissues. Functionally, hsa_circ_0063865 promoted malignant transformation of Het-1A cells induced by NMBzA and MC-LR by promoting cytoskeletal rearrangements and inhibiting ER stress of MYH9
conclusionThese findings identify a novel chemical carcinogenic target and elucidate the dual-regulatory mechanism of hsa_circ_0063865 in NAs and MC-LR-induced esophageal carcinogenesis.
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